6ESU
Artificial imine reductase mutant S112A-N118P-K121A-S122M
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Streptavidin | polymer | 159 | 16659.2 | 1 | UniProt (P22629) Pfam (PF01382) In PDB | Streptomyces avidinii | |
2 | A | 5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-[4-(2-azanylethylsulfamoyl)phenyl]pentanamide | non-polymer | 441.6 | 1 | Chemie (6IR) | |||
3 | A | IRIDIUM ION | non-polymer | 192.2 | 4 | Chemie (IR) | |||
4 | A | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) | |||
5 | A | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
6 | water | water | 18.0 | 29 | Chemie (HOH) |
Sequence modifications
A: 14 - 159 (UniProt: P22629)
PDB | External Database | Details |
---|---|---|
Met 1 | - | initiating methionine |
Ala 2 | - | expression tag |
Ser 3 | - | expression tag |
Met 4 | - | expression tag |
Thr 5 | - | expression tag |
Gly 6 | - | expression tag |
Gly 7 | - | expression tag |
Gln 8 | - | expression tag |
Gln 9 | - | expression tag |
Met 10 | - | expression tag |
Gly 11 | - | expression tag |
Arg 12 | - | expression tag |
Asp 13 | - | expression tag |
Arg 112 | Ser 136 | engineered mutation |
Pro 118 | Asn 142 | engineered mutation |
Ala 121 | Lys 145 | engineered mutation |
Met 122 | Ser 146 | engineered mutation |
Tyr 124 | Leu 148 | conflict |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 16659.2 | |
Non-Polymers* | Number of molecules | 7 |
Total formula weight | 1365.5 | |
All* | Total formula weight | 18024.7 |