6ERA

Crystal structure of cyclohexanone monooxygenase mutant (F249A, F280A and F435A) from Rhodococcus sp. Phi1 bound to NADP+

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Cyclohexanone monooxygenase	polymer	549	60711.4	2	UniProt (Q84H73) Pfam (PF00743) In PDB	Rhodococcus sp. Phi1
2	A, B	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	2	Chemie (FAD)
3	A, B	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		743.4	2	Chemie (NAP)
4		water	water		18.0	137	Chemie (HOH)

Sequence modifications

A, B: 1 - 541 (UniProt: Q84H73)

PDB	External Database	Details
Ala 249	Phe 249	engineered mutation
Ala 280	Phe 280	engineered mutation
Ala 435	Phe 435	engineered mutation
Leu 542	-	expression tag
Glu 543	-	expression tag
His 544	-	expression tag
His 545	-	expression tag
His 546	-	expression tag
His 547	-	expression tag
His 548	-	expression tag
His 549	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	121422.9
Non-Polymers*	Number of molecules	4
	Total formula weight	3057.9
All*	Total formula weight	124480.8

*Water molecules are not included.