6ERA
Crystal structure of cyclohexanone monooxygenase mutant (F249A, F280A and F435A) from Rhodococcus sp. Phi1 bound to NADP+
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Cyclohexanone monooxygenase | polymer | 549 | 60711.4 | 2 | UniProt (Q84H73) Pfam (PF00743) | Rhodococcus sp. Phi1 | |
| 2 | C, E (A, B) | FLAVIN-ADENINE DINUCLEOTIDE | non-polymer | 785.5 | 2 | Chemie (FAD) | |||
| 3 | D, F (A, B) | NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 743.4 | 2 | Chemie (NAP) | |||
| 4 | G, H (A, B) | water | water | 18.0 | 137 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 541 (UniProt: Q84H73)
| PDB | External Database | Details |
|---|---|---|
| Ala 249 | Phe 249 | engineered mutation |
| Ala 280 | Phe 280 | engineered mutation |
| Ala 435 | Phe 435 | engineered mutation |
| Leu 542 | - | expression tag |
| Glu 543 | - | expression tag |
| His 544 | - | expression tag |
| His 545 | - | expression tag |
| His 546 | - | expression tag |
| His 547 | - | expression tag |
| His 548 | - | expression tag |
| His 549 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 121422.9 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 3057.9 | |
| All* | Total formula weight | 124480.8 |
