6EQZ

A MamC-MIC insertion in MBP scaffold at position K170

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, D, G)	Maltose-binding periplasmic protein,Tightly bound bacterial magnetic particle protein,Maltose-binding periplasmic protein	polymer	401	43833.5	4	UniProt (P0AEY0) UniProt (Q2W8S0) Pfam (PF01547) UniProt (by SIFTS) (P0AEX9)	Escherichia coli O157:H7	MBP,MMBP,Maltodextrin-binding protein,MBP,MMBP,Maltodextrin-binding protein
2	E, F, G, H (C, E, F, H)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	4	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
3	I, J, K, L (A, B, D, G)	water	water		18.0	288	Chemie (HOH)

Sequence modifications

A, B, D, G: 1 - 169 (UniProt: P0AEY0)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag

A, B, D, G: 170 - 190 (UniProt: Q2W8S0)
A, B, D, G: 191 - 376 (UniProt: P0AEY0)

PDB	External Database	Details
Asn 377	-	expression tag
Ser 378	-	expression tag
Ser 379	-	expression tag
Ser 380	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	175333.9
Branched	Number of molecules	4
	Total formula weight	1369.2
All*	Total formula weight	176703.0

*Water molecules are not included.