6EMT

Crystal Structure of dual specific Trm10 construct from Thermococcus kodakaraensis.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	tRNA (guanine(9)-/adenine(9)-N1)-methyltransferase	polymer	197	22422.6	1	UniProt (Q5JD38) In PDB	Thermococcus kodakarensis (Pyrococcus kodakaraensis)	tRNA(m1G9/m1A9)-methyltransferase,tRNA(m1G9/m1A9)MTase
2	A	ACETATE ION	non-polymer		59.0	8	Chemie (ACT)
3	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	A	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
5		water	water		18.0	113	Chemie (HOH)

Sequence modifications

A: 97 - 272 (UniProt: Q5JD38)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Met 96	-	expression tag
Ala 120	Cys 120	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	22422.6
Non-Polymers*	Number of molecules	11
	Total formula weight	778.7
All*	Total formula weight	23201.3

*Water molecules are not included.