6EMT

Crystal Structure of dual specific Trm10 construct from Thermococcus kodakaraensis.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-23
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 61 2 2
Unit cell lengths	62.262, 62.262, 203.510
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.645 - 1.794
R-factor	0.1921
Rwork	0.191
R-free	0.20620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SPOUT domain of PDB ID 6EMS
RMSD bond length	0.003
RMSD bond angle	0.515
Data reduction software	XDS (2015-10-15)
Data scaling software	XDS (2015-10-15)
Phasing software	PHASER (2.5.5)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.645	1.900
High resolution limit [Å]	1.794	1.800
Rmerge	0.091	2.361
Rmeas	0.092	2.394
Rpim	0.015	0.387
Number of reflections	22813	2160
<I/σ(I)>	30.85	1.63
Completeness [%]	99.8	97.87
Redundancy	37.9	36.5
CC(1/2)	1.000	0.763

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5	298	20 % PEG 3350, 8% v/v Tascimate