6EKP

Tryptophan Repressor TrpR from E.coli variant T44L T81M S88Y with Indole-3-acetic acid as ligand

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Trp operon repressor	polymer	116	13559.5	2	UniProt (P0A881) Pfam (PF01371) In PDB	Escherichia coli (strain K12)
2	A, B	1H-INDOL-3-YLACETIC ACID	non-polymer		175.2	2	Chemie (IAC)
3	A	3,6,9,12,15-PENTAOXAHEPTADECANE	non-polymer		250.3	1	Chemie (P3G)
4		water	water		18.0	250	Chemie (HOH)

Sequence modifications

A, B: 1 - 108 (UniProt: P0A881)

PDB	External Database	Details
Leu 44	Thr 44	engineered mutation
Met 81	Thr 81	engineered mutation
Tyr 88	Ser 88	engineered mutation
Leu 109	-	expression tag
Glu 110	-	expression tag
His 111	-	expression tag
His 112	-	expression tag
His 113	-	expression tag
His 114	-	expression tag
His 115	-	expression tag
His 116	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	27119.0
Non-Polymers*	Number of molecules	3
	Total formula weight	600.7
All*	Total formula weight	27719.8

*Water molecules are not included.