6EI8

Crystal structure of human tRNA-dihydrouridine (20) synthase dsRBD F359A mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	tRNA-dihydrouridine(20) synthase [NAD(P)+]-like	polymer	120	13652.6	1	UniProt (Q9NX74) Pfam (PF00035) In PDB	Homo sapiens (Human)	Dihydrouridine synthase 2,Up-regulated in lung cancer protein 8,URLC8,tRNA-dihydrouridine synthase 2-like,hDUS2
2	A	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
3		water	water		18.0	48	Chemie (HOH)

Sequence modifications

A: 338 - 450 (UniProt: Q9NX74)

PDB	External Database	Details
Met 337	-	initiating methionine
Ala 359	Phe 359	engineered mutation
His 451	-	expression tag
His 452	-	expression tag
His 453	-	expression tag
His 454	-	expression tag
His 455	-	expression tag
His 456	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	13652.6
Non-Polymers*	Number of molecules	3
	Total formula weight	288.2
All*	Total formula weight	13940.8

*Water molecules are not included.