6EI8

Crystal structure of human tRNA-dihydrouridine (20) synthase dsRBD F359A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9762
Spacegroup name	P 31 2 1
Unit cell lengths	81.110, 81.110, 56.050
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.810 - 2.250
R-factor	0.262
Rwork	0.259
R-free	0.28600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wft
RMSD bond length	0.010
RMSD bond angle	1.080
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.810	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.233
Number of reflections	10384	1020
<I/σ(I)>	9.34	1.01
Completeness [%]	99.9	100
Redundancy	11.2	11.6
CC(1/2)	0.999	0.746

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	292	2M ammonium sulfate 50 mM sodium cacodylate 15 mM magnesium acetate