6EHS

E. coli Hydrogenase-2 chemically reduced structure

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (S, T)	Hydrogenase-2 small chain	polymer	300	32631.8	2	UniProt (P69741) Pfam (PF01058) Pfam (PF14720) UniProt (by SIFTS) (B7LGE4)	Escherichia coli	HYD2,Membrane-bound hydrogenase 2 small subunit,NiFe hydrogenase
2	B, D (L, M)	Hydrogenase-2 large chain	polymer	552	61048.2	2	UniProt (P0ACE0) Pfam (PF00374) UniProt (by SIFTS) (P0ACE1)	Escherichia coli (strain K12)	HYD2,Membrane-bound hydrogenase 2 large subunit,NiFe hydrogenase
3	E, G, M, O (S, T)	IRON/SULFUR CLUSTER	non-polymer		351.6	4	Chemie (SF4)
4	F, N (S, T)	FE3-S4 CLUSTER	non-polymer		295.8	2	Chemie (F3S)
5	H, P (L, M)	CARBONMONOXIDE-(DICYANO) IRON	non-polymer		135.9	2	Chemie (FCO)
6	I, Q (L, M)	NICKEL (II) ION	non-polymer		58.7	2	Chemie (NI)
7	J, R (L, M)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
8	K, L, S, T (L, M)	DITHIONITE	non-polymer		128.1	4	Chemie (DTN)
9	U, V, W, X (S, L, T, M)	water	water		18.0	999	Chemie (HOH)

Sequence modifications

S, T: 1 - 293 (UniProt: P69741)

PDB	External Database	Details
Met 0	-	initiating methionine
His 294	-	expression tag
His 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag
His 298	-	expression tag
His 299	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	187359.9
Non-Polymers*	Number of molecules	16
	Total formula weight	2948.4
All*	Total formula weight	190308.4

*Water molecules are not included.