6EHS

E. coli Hydrogenase-2 chemically reduced structure

Functional Information from GO Data

Chain	GOid	namespace	contents
L	0005886	cellular_component	plasma membrane
L	0008901	molecular_function	ferredoxin hydrogenase activity
L	0016151	molecular_function	nickel cation binding
L	0016491	molecular_function	oxidoreductase activity
L	0033748	molecular_function	hydrogenase (acceptor) activity
L	0046872	molecular_function	metal ion binding
M	0005886	cellular_component	plasma membrane
M	0008901	molecular_function	ferredoxin hydrogenase activity
M	0016151	molecular_function	nickel cation binding
M	0016491	molecular_function	oxidoreductase activity
M	0033748	molecular_function	hydrogenase (acceptor) activity
M	0046872	molecular_function	metal ion binding
S	0008901	molecular_function	ferredoxin hydrogenase activity
S	0009375	cellular_component	ferredoxin hydrogenase complex
S	0051536	molecular_function	iron-sulfur cluster binding
T	0008901	molecular_function	ferredoxin hydrogenase activity
T	0009375	cellular_component	ferredoxin hydrogenase complex
T	0051536	molecular_function	iron-sulfur cluster binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	9
Details	binding site for residue SF4 S 301

Chain	Residue
S	HIS192
S	CYS195
S	ARG197
S	ARG198
S	CYS220
S	LEU221
S	TYR222
S	CYS226
S	GLY228

---

site_id	AC2
Number of Residues	9
Details	binding site for residue F3S S 302

Chain	Residue
L	LYS211
L	GLN216
S	CYS235
S	PHE240
S	CYS255
S	TYR256
S	GLY257
S	CYS258
S	ASN259

---

site_id	AC3
Number of Residues	8
Details	binding site for residue SF4 S 303

Chain	Residue
L	ARG59
S	CYS22
S	CYS25
S	SER119
S	CYS120
S	GLY153
S	CYS154
S	PRO155

---

site_id	AC4
Number of Residues	13
Details	binding site for residue FCO L 601

Chain	Residue
L	CYS64
L	THR67
L	HIS68
L	ALA477
L	PRO478
L	ARG479
L	LEU482
L	VAL500
L	PRO501
L	SER502
L	CYS549
L	NI602
L	HOH1014

---

site_id	AC5
Number of Residues	6
Details	binding site for residue NI L 602

Chain	Residue
L	CYS61
L	CYS64
L	CSO546
L	CYS549
L	FCO601
L	HOH1014

---

site_id	AC6
Number of Residues	6
Details	binding site for residue MG L 603

Chain	Residue
L	GLU42
L	ALA498
L	HIS552
L	HOH720
L	HOH732
L	HOH737

---

site_id	AC7
Number of Residues	6
Details	binding site for residue DTN L 604

Chain	Residue
L	GLU196
L	ARG199
L	ARG203
L	HOH758
M	LYS249
S	HOH444

---

site_id	AC8
Number of Residues	5
Details	binding site for residue DTN L 605

Chain	Residue
L	LEU298
L	ARG391
L	ASN441
L	HOH702
L	HOH716

---

site_id	AC9
Number of Residues	8
Details	binding site for residue SF4 T 301

Chain	Residue
T	HIS192
T	CYS195
T	ARG197
T	ARG198
T	CYS220
T	LEU221
T	TYR222
T	CYS226

---

site_id	AD1
Number of Residues	9
Details	binding site for residue F3S T 302

Chain	Residue
M	LYS211
M	GLN216
T	CYS235
T	PHE240
T	CYS255
T	TYR256
T	GLY257
T	CYS258
T	ASN259

---

site_id	AD2
Number of Residues	9
Details	binding site for residue SF4 T 303

Chain	Residue
M	ARG59
T	CYS22
T	CYS25
T	SER119
T	CYS120
T	GLY153
T	CYS154
T	PRO155
T	HOH404

---

site_id	AD3
Number of Residues	12
Details	binding site for residue FCO M 601

Chain	Residue
M	HOH976
M	CYS64
M	HIS68
M	ALA477
M	PRO478
M	ARG479
M	LEU482
M	VAL500
M	PRO501
M	SER502
M	CYS549
M	NI602

---

site_id	AD4
Number of Residues	6
Details	binding site for residue NI M 602

Chain	Residue
M	CYS61
M	CYS64
M	CSO546
M	CYS549
M	FCO601
M	HOH976

---

site_id	AD5
Number of Residues	6
Details	binding site for residue MG M 603

Chain	Residue
M	GLU42
M	ALA498
M	HIS552
M	HOH713
M	HOH733
M	HOH758

---

site_id	AD6
Number of Residues	8
Details	binding site for residue DTN M 604

Chain	Residue
L	LYS249
M	GLU196
M	ARG199
M	ARG203
M	HOH707
M	HOH715
M	HOH787
T	THR33

---

site_id	AD7
Number of Residues	4
Details	binding site for residue DTN M 605

Chain	Residue
M	ARG391
M	ASN441
M	HOH704
M	HOH893

---

Functional Information from PROSITE/UniProt

site_id	PS00507
Number of Residues	26
Details	NI_HGENASE_L_1 Nickel-dependent hydrogenases large subunit signature 1. RGMEeivknrdprdawmivQRiCGVC

Chain	Residue	Details
L	ARG39-CYS64

---

site_id	PS00508
Number of Residues	10
Details	NI_HGENASE_L_2 Nickel-dependent hydrogenases large subunit signature 2. FDPCMACav.H

Chain	Residue	Details
L	PHE543-HIS552

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	BINDING: BINDING => ECO:0000255

Chain	Residue	Details
L	CYS61
S	CYS255
S	CYS258
T	CYS22
T	CYS25
T	CYS120
T	CYS154
T	HIS192
T	CYS195
T	CYS220
T	CYS226
L	CYS64
T	CYS235
T	CYS255
T	CYS258
L	CSO546
L	CYS549
M	CYS61
M	CYS64
M	CSO546
M	CYS549
S	CYS235

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	SITE: Cleavage; by HybD => ECO:0000250

Chain	Residue	Details
L	HIS552
M	HIS552

---