6EEC

Mycobacterium tuberculosis RNAP promoter unwinding intermediate complex with RbpA/CarD and AP3 promoter captured by Corallopyronin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA-directed RNA polymerase subunit alpha	polymer	347	37745.3	2	UniProt (A5U8D3) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (P9WGZ1)	Mycobacterium tuberculosis	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C (C)	DNA-directed RNA polymerase subunit beta	polymer	1179	130070.8	1	UniProt (V9Z879) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (P9WGY9)	Mycobacterium tuberculosis	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D (D)	DNA-directed RNA polymerase subunit beta'	polymer	1326	148202.2	1	UniProt (A5U053) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P9WGY7)	Mycobacterium tuberculosis	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E (E)	DNA-directed RNA polymerase subunit omega	polymer	110	11777.0	1	UniProt (A0A0T9N9K3) Pfam (PF01192) UniProt (by SIFTS) (P9WGY5)	Mycobacterium tuberculosis	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F (F)	RNA polymerase sigma factor SigA	polymer	531	58169.5	1	UniProt (P9WGI0) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) UniProt (by SIFTS) (P9WGI1)	Mycobacterium tuberculosis	Sigma-A
6	G (J)	RNA polymerase-binding protein RbpA	polymer	111	12993.7	1	UniProt (P9WHJ4) Pfam (PF13397) UniProt (by SIFTS) (P9WHJ5)	Mycobacterium tuberculosis
7	H (O)	DNA (65-MER)	polymer	90	27907.8	1		Mycobacterium tuberculosis
8	I (P)	DNA (63-MER)	polymer	90	27618.6	1		Mycobacterium tuberculosis
9	J (M)	RNA polymerase-binding transcription factor CarD	polymer	162	17933.4	1	UniProt (P9WJG2) Pfam (PF02559) Pfam (PF21095) UniProt (by SIFTS) (P9WJG3)	Mycobacterium tuberculosis
10	K (C)	methyl [(1E,5R)-5-{(3E)-3-[(2E,4E,8R,9E,12E)-1,8-dihydroxy-2,5,9-trimethyltetradeca-2,4,9,12-tetraen-1-ylidene]-2,4-dioxo-3,4-d ihydro-2H-pyran-6-yl}hex-1-en-1-yl]carbamate	non-polymer		527.6	1	Chemie (C0L)
11	L, M (D)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
12	N (D)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

C: 7 - 1178 (UniProt: V9Z879)

PDB	External Database	Details
Leu 1179	-	expression tag
Ala 1180	-	expression tag
Arg 1181	-	expression tag
His 1182	-	expression tag
Gly 1183	-	expression tag
Gly 1184	-	expression tag
Ser 1185	-	expression tag

D: 1 - 1316 (UniProt: A5U053)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag
His 1317	-	expression tag
His 1318	-	expression tag
His 1319	-	expression tag
His 1320	-	expression tag
His 1321	-	expression tag
His 1322	-	expression tag
His 1323	-	expression tag
His 1324	-	expression tag

E: 2 - 110 (UniProt: A0A0T9N9K3)

PDB	External Database	Details
Gly 1	-	expression tag

F: 1 - 528 (UniProt: P9WGI0)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	510163.6
Non-Polymers*	Number of molecules	4
	Total formula weight	682.8
All*	Total formula weight	510846.4

*Water molecules are not included.