6EBS

Crystal structure of Leishmania major dihydroorotate dehydrogenase mutant H174A in complex with orotate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Dihydroorotate dehydrogenase (fumarate)	polymer	354	38092.5	2	UniProt (Q4QEW7) Pfam (PF01180)	Leishmania major	Dihydroorotate oxidase
2	C, K (A, B)	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	2	Chemie (FMN)
3	D, L (A, B)	OROTIC ACID	non-polymer		156.1	2	Chemie (ORO)
4	E, F, G, M (A, B)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
5	H, I, J, N, O... (A, B)	SULFATE ION	non-polymer		96.1	6	Chemie (SO4)
6	Q (B)	NICKEL (II) ION	non-polymer		58.7	1	Chemie (NI)
7	R, S (A, B)	water	water		18.0	496	Chemie (HOH)

Sequence modifications

A, B: 1 - 320 (UniProt: Q4QEW7)

PDB	External Database	Details
Met -33	-	initiating methionine
Gly -32	-	expression tag
Ser -31	-	expression tag
Ser -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
Ser -23	-	expression tag
Ser -22	-	expression tag
Gly -21	-	expression tag
Leu -20	-	expression tag
Val -19	-	expression tag
Pro -18	-	expression tag
Ala -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
Met -13	-	expression tag
Ala -12	-	expression tag
Ser -11	-	expression tag
Met -10	-	expression tag
Thr -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Gln -6	-	expression tag
Gln -5	-	expression tag
Met -4	-	expression tag
Gly -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 174	His 174	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	76184.9
Non-Polymers*	Number of molecules	15
	Total formula weight	2228.3
All*	Total formula weight	78413.2

*Water molecules are not included.