6E4E

Crystal structure of dihydrofolate reductase from Staphylococcus aureus MW2 bound to NADP and p218

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Dihydrofolate reductase	polymer	182	20736.7	1	UniProt (P0A017) Pfam (PF00186)	Staphylococcus aureus	DHFR
2	B (A)	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		743.4	1	Chemie (NAP)
3	C (A)	3-(2-{3-[(2,4-diamino-6-ethylpyrimidin-5-yl)oxy]propoxy}phenyl)propanoic acid	non-polymer		360.4	1	Chemie (MMV)
4	D (A)	water	water		18.0	117	Chemie (HOH)

Sequence modifications

A: 0 - 158 (UniProt: P0A017)

PDB	External Database	Details
Met -23	-	initiating methionine
Gly -22	-	expression tag
Ser -21	-	expression tag
Ser -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
Ser -13	-	expression tag
Ser -12	-	expression tag
Gly -11	-	expression tag
Leu -10	-	expression tag
Val -9	-	expression tag
Pro -8	-	expression tag
Arg -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
His -4	-	expression tag
Met -3	-	expression tag
Ala -2	-	expression tag
Ser -1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	20736.7
Non-Polymers*	Number of molecules	2
	Total formula weight	1103.8
All*	Total formula weight	21840.5

*Water molecules are not included.