6DXS
Crystal structure of the LigJ hydratase E284Q mutant substrate complex with (3Z)-2-keto-4-carboxy-3-hexenedioate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | 4-oxalomesaconate hydratase | polymer | 342 | 38203.5 | 4 | UniProt (G2IQQ5) Pfam (PF04909) | Sphingobium sp. SYK-6 | |
| 2 | E, G, I, K (A, B, C, D) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
| 3 | F, H, J, L (A, B, C, D) | (2Z)-4-oxobut-2-ene-1,2,4-tricarboxylic acid | non-polymer | 202.1 | 4 | Chemie (HJ7) | |||
| 4 | M, N, O, P (A, B, C, D) | water | water | 18.0 | 737 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 2 - 341 (UniProt: G2IQQ5)
| PDB | External Database | Details |
|---|---|---|
| Gln 284 | Glu 284 | engineered mutation |
| Leu 342 | - | expression tag |
| Glu 343 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 152814.0 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 1070.1 | |
| All* | Total formula weight | 153884.1 |
