6DXS
Crystal structure of the LigJ hydratase E284Q mutant substrate complex with (3Z)-2-keto-4-carboxy-3-hexenedioate
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0016829 | molecular_function | lyase activity |
| A | 0016831 | molecular_function | carboxy-lyase activity |
| A | 0019439 | biological_process | obsolete aromatic compound catabolic process |
| A | 0019748 | biological_process | secondary metabolic process |
| A | 0046274 | biological_process | lignin catabolic process |
| A | 0046872 | molecular_function | metal ion binding |
| A | 1901360 | biological_process | organic cyclic compound metabolic process |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0016829 | molecular_function | lyase activity |
| B | 0016831 | molecular_function | carboxy-lyase activity |
| B | 0019439 | biological_process | obsolete aromatic compound catabolic process |
| B | 0019748 | biological_process | secondary metabolic process |
| B | 0046274 | biological_process | lignin catabolic process |
| B | 0046872 | molecular_function | metal ion binding |
| B | 1901360 | biological_process | organic cyclic compound metabolic process |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0016787 | molecular_function | hydrolase activity |
| C | 0016829 | molecular_function | lyase activity |
| C | 0016831 | molecular_function | carboxy-lyase activity |
| C | 0019439 | biological_process | obsolete aromatic compound catabolic process |
| C | 0019748 | biological_process | secondary metabolic process |
| C | 0046274 | biological_process | lignin catabolic process |
| C | 0046872 | molecular_function | metal ion binding |
| C | 1901360 | biological_process | organic cyclic compound metabolic process |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0016787 | molecular_function | hydrolase activity |
| D | 0016829 | molecular_function | lyase activity |
| D | 0016831 | molecular_function | carboxy-lyase activity |
| D | 0019439 | biological_process | obsolete aromatic compound catabolic process |
| D | 0019748 | biological_process | secondary metabolic process |
| D | 0046274 | biological_process | lignin catabolic process |
| D | 0046872 | molecular_function | metal ion binding |
| D | 1901360 | biological_process | organic cyclic compound metabolic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | binding site for residue ZN A 401 |
| Chain | Residue |
| A | HIS8 |
| A | HIS10 |
| A | HIS178 |
| A | GLN284 |
| A | HJ7402 |
| site_id | AC2 |
| Number of Residues | 15 |
| Details | binding site for residue HJ7 A 402 |
| Chain | Residue |
| A | ALA72 |
| A | SER73 |
| A | THR190 |
| A | TYR194 |
| A | HIS223 |
| A | GLN284 |
| A | ALA288 |
| A | ARG290 |
| A | ZN401 |
| A | HOH556 |
| D | ARG234 |
| A | HIS8 |
| A | HIS10 |
| A | THR12 |
| A | ARG71 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | binding site for residue ZN B 401 |
| Chain | Residue |
| B | HIS8 |
| B | HIS10 |
| B | HIS178 |
| B | HJ7402 |
| site_id | AC4 |
| Number of Residues | 17 |
| Details | binding site for residue HJ7 B 402 |
| Chain | Residue |
| B | HIS8 |
| B | HIS10 |
| B | THR12 |
| B | ARG71 |
| B | ALA72 |
| B | SER73 |
| B | HIS178 |
| B | TYR194 |
| B | HIS223 |
| B | GLN284 |
| B | GLY287 |
| B | ALA288 |
| B | ARG290 |
| B | ZN401 |
| B | HOH538 |
| B | HOH553 |
| C | ARG234 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | binding site for residue ZN C 401 |
| Chain | Residue |
| C | HIS8 |
| C | HIS10 |
| C | HIS178 |
| C | HJ7402 |
| site_id | AC6 |
| Number of Residues | 17 |
| Details | binding site for residue HJ7 C 402 |
| Chain | Residue |
| B | ARG234 |
| C | HIS8 |
| C | HIS10 |
| C | THR12 |
| C | ARG71 |
| C | ALA72 |
| C | SER73 |
| C | HIS178 |
| C | TYR194 |
| C | HIS223 |
| C | GLN284 |
| C | GLY287 |
| C | ALA288 |
| C | ARG290 |
| C | ZN401 |
| C | HOH531 |
| C | HOH564 |
| site_id | AC7 |
| Number of Residues | 4 |
| Details | binding site for residue ZN D 401 |
| Chain | Residue |
| D | HIS8 |
| D | HIS10 |
| D | HIS178 |
| D | HJ7402 |
| site_id | AC8 |
| Number of Residues | 15 |
| Details | binding site for residue HJ7 D 402 |
| Chain | Residue |
| A | ARG234 |
| D | HIS8 |
| D | HIS10 |
| D | THR12 |
| D | ARG71 |
| D | ALA72 |
| D | SER73 |
| D | HIS178 |
| D | TYR194 |
| D | HIS223 |
| D | GLN284 |
| D | ARG290 |
| D | ZN401 |
| D | HOH548 |
| D | HOH573 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton donor/acceptor => ECO:0000305|PubMed:30207699 |
| Chain | Residue | Details |
| A | GLN284 | |
| B | GLN284 | |
| C | GLN284 | |
| D | GLN284 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:30207699, ECO:0000312|PDB:6DWV |
| Chain | Residue | Details |
| A | HIS8 | |
| A | HIS10 | |
| A | HIS178 | |
| B | HIS8 | |
| B | HIS10 | |
| B | HIS178 | |
| C | HIS8 | |
| C | HIS10 | |
| C | HIS178 | |
| D | HIS8 | |
| D | HIS10 | |
| D | HIS178 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 16 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:30207699, ECO:0000312|PDB:6DXS |
| Chain | Residue | Details |
| D | ARG71 | |
| D | TYR194 | |
| D | HIS223 | |
| D | ARG290 | |
| A | ARG71 | |
| A | TYR194 | |
| A | HIS223 | |
| A | ARG290 | |
| B | ARG71 | |
| B | TYR194 | |
| B | HIS223 | |
| B | ARG290 | |
| C | ARG71 | |
| C | TYR194 | |
| C | HIS223 | |
| C | ARG290 |
