6DW0

Cryo-EM structure of the benzodiazepine-sensitive alpha1beta1gamma2S tri-heteromeric GABAA receptor in complex with GABA (Whole map)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (D)	Gamma-aminobutyric acid receptor subunit gamma-2	polymer	490	56894.9	1	UniProt (P18508) Pfam (PF02931) Pfam (PF02932)	Rattus norvegicus (Rat)	GABA(A) receptor subunit gamma-2
2	B, C (C, A)	Gamma-aminobutyric acid receptor subunit alpha-1,Gamma-aminobutyric acid receptor subunit alpha-1	polymer	402	46267.1	2	UniProt (P62813) Pfam (PF02931) Pfam (PF02932)	Rattus norvegicus (Rat)	GABA(A) receptor subunit alpha-1,GABA(A) receptor subunit alpha-1
3	D, E (E, B)	Gamma-aminobutyric acid receptor subunit beta-1,Gamma-aminobutyric acid receptor subunit beta-1	polymer	384	44333.0	2	UniProt (P15431) Pfam (PF02931) Pfam (PF02932)	Rattus norvegicus (Rat)	GABA(A) receptor subunit beta-1,GABA(A) receptor subunit beta-1
4	F (F)	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1	In PDB GlyTouCan (G81315DD)
5	G (G)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1235.1	1	In PDB GlyTouCan (G07617FP)
6	H, J (H, J)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	2	In PDB GlyTouCan (G15407YE)
7	I, K (I, K)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
8	L, M, N (A, B)	GAMMA-AMINO-BUTANOIC ACID	non-polymer		103.1	3	Chemie (ABU)

Sequence modifications

D: -37 - 428 (UniProt: P18508)

PDB	External Database	Details
Leu 429	-	expression tag
Val 430	-	expression tag
Pro 431	-	expression tag
Arg 432	-	expression tag
Gly 433	-	expression tag
Ser 434	-	expression tag
Arg 435	-	expression tag
His 436	-	expression tag
His 437	-	expression tag
His 438	-	expression tag
His 439	-	expression tag
His 440	-	expression tag
His 441	-	expression tag
His 442	-	expression tag
His 443	-	expression tag
Thr 444	-	expression tag
Glu 445	-	expression tag
Thr 446	-	expression tag
Ser 447	-	expression tag
Gln 448	-	expression tag
Val 449	-	expression tag
Ala 450	-	expression tag
Pro 451	-	expression tag
Ala 452	-	expression tag

C, A: -26 - 313 (UniProt: P62813)
C, A: 315 - 361 (UniProt: P62813)

PDB	External Database	Details
Thr 314	-	linker
Leu 362	-	expression tag
Val 363	-	expression tag
Pro 364	-	expression tag
Arg 365	-	expression tag
Gly 366	-	expression tag
Ser 367	-	expression tag
His 368	-	expression tag
His 369	-	expression tag
His 370	-	expression tag
His 371	-	expression tag
His 372	-	expression tag
His 373	-	expression tag
His 374	-	expression tag
His 375	-	expression tag

E, B: -24 - 308 (UniProt: P15431)
E, B: 311 - 345 (UniProt: P15431)

PDB	External Database	Details
Gly 309	-	linker
Thr 310	-	linker
Leu 346	-	expression tag
Val 347	-	expression tag
Pro 348	-	expression tag
Arg 349	-	expression tag
Gly 350	-	expression tag
Ser 351	-	expression tag
His 352	-	expression tag
His 353	-	expression tag
His 354	-	expression tag
His 355	-	expression tag
His 356	-	expression tag
His 357	-	expression tag
His 358	-	expression tag
His 359	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	238095.2
Branched	Number of molecules	6
	Total formula weight	4005.7
Non-Polymers*	Number of molecules	3
	Total formula weight	309.4
All*	Total formula weight	242410.2

*Water molecules are not included.