6DUQ

Structure of a Rho-NusG KOW domain complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (L, A, G, B, H...)	Transcription termination factor Rho	polymer	422	47396.6	12	UniProt (F4TMM7) Pfam (PF07498) Pfam (PF07497) Pfam (PF00006) UniProt (by SIFTS) (P0AG30)	Escherichia coli M718	ATP-dependent helicase Rho
2	M, N, O, P, Q... (M, N, O, P, Q...)	Transcription termination/antitermination protein NusG	polymer	60	6657.4	12	UniProt (F4T6L5) Pfam (PF00467) UniProt (by SIFTS) (P0AFG0)	Escherichia coli M605
3	Y, Z (Y, Z)	rU12	polymer	12	3629.0	2		synthetic construct
4	AA, BB, DA, EB, GA... (L, F, A, J, G...)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	12	Chemie (ADP)
5	BA, CB, EA, FB, HA... (L, F, A, J, G...)	MAGNESIUM ION	non-polymer		24.3	12	Chemie (MG)
6	AB, CA, DB, FA, GB... (K, L, F, A, J...)	BERYLLIUM TRIFLUORIDE ION	non-polymer		66.0	12	Chemie (BEF)

Sequence modifications

L, A, G, B, H, C, I, D, K, F, J, E: 1 - 419 (UniProt: F4TMM7)

PDB	External Database	Details
Met -2	-	initiating methionine
Gly -1	-	expression tag
His 0	-	expression tag

M, N, O, P, Q, R, S, T, U, V, W, X: 125 - 181 (UniProt: F4T6L5)

PDB	External Database	Details
Ser 122	-	expression tag
Asn 123	-	expression tag
Ala 124	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	26
	Total formula weight	655905.7
Non-Polymers*	Number of molecules	36
	Total formula weight	6210.2
All*	Total formula weight	662115.8

*Water molecules are not included.