6DPM

Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with an RNA/DNA Hybrid: Reaction in 10 mM Mg2+ and 200 mM Rb+ for 1800 s at 21 C

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ribonuclease H	polymer	142	16272.4	1	UniProt (Q9KEI9)	Bacillus halodurans	RNase H
2	B (B)	5'-R(*AP*CP*AP*U)-3' portion of cleaved RNA 5'-R(*AP*CP*AP*UP*CP*G)-3'	polymer	4	1224.8	1		synthetic construct
3	C (b)	5'-R(P*CP*G)-3' portion of cleaved RNA (5'-R(*AP*CP*AP*UP*CP*G)-3')	polymer	2	605.4	1		synthetic construct
4	D (C)	DNA (5'-D(*CP*GP*AP*TP*GP*T)-3')	polymer	6	1824.2	1		synthetic construct
5	E, F (A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	G, H, I, J, T... (A, B, C)	RUBIDIUM ION	non-polymer		85.5	6	Chemie (RB)
7	K, L, M (A)	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
8	N, O, P, V (A, C)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
9	Q, R, S, W (A, C)	1,2-ETHANEDIOL	non-polymer		62.1	4	Chemie (EDO)
10	AA, X, Y, Z (C, A, B, b)	water	water		18.0	181	Chemie (HOH)

Sequence modifications

A: 59 - 196 (UniProt: Q9KEI9)

PDB	External Database	Details
Gly 55	-	expression tag
Ser 56	-	expression tag
His 57	-	expression tag
Met 58	-	expression tag
Ala 196	Lys 196	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	19926.8
Non-Polymers*	Number of molecules	19
	Total formula weight	1284.4
All*	Total formula weight	21211.2

*Water molecules are not included.