6DPM
Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with an RNA/DNA Hybrid: Reaction in 10 mM Mg2+ and 200 mM Rb+ for 1800 s at 21 C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.843, 37.454, 62.728 |
| Unit cell angles | 90.00, 97.69, 90.00 |
Refinement procedure
| Resolution | 20.086 - 1.677 |
| R-factor | 0.1616 |
| Rwork | 0.160 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 1ZBL |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.988 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.090 | 20.090 | 1.710 |
| High resolution limit [Å] | 1.680 | 9.030 | 1.680 |
| Rmerge | 0.048 | 0.019 | 0.732 |
| Rmeas | 0.068 | 0.027 | 1.035 |
| Rpim | 0.048 | 0.019 | 0.732 |
| Total number of observations | 41867 | ||
| Number of reflections | 21641 | 140 | 909 |
| <I/σ(I)> | 9 | ||
| Completeness [%] | 98.9 | 90.9 | 80.2 |
| Redundancy | 1.9 | 1.7 | 1.9 |
| CC(1/2) | 0.998 | 0.998 | 0.467 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2 |
