6DPK

Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with an RNA/DNA Hybrid: Reaction in 4 mM Mn2+ and 200 mM K+ for 240 s at 21 C

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ribonuclease H	polymer	142	16272.4	1	UniProt (Q9KEI9)	Bacillus halodurans	RNase H
2	B (B)	5'-R(*AP*CP*AP*U)-3' portion of cleaved RNA 5'-R(*AP*CP*AP*UP*CP*G)-3'	polymer	4	1224.8	1		synthetic construct
3	C (b)	5'-R(P*CP*G)-3' portion of cleaved RNA (5'-R(*AP*CP*AP*UP*CP*G)-3')	polymer	2	605.4	1		synthetic construct
4	D (C)	DNA (5'-D(*CP*GP*AP*TP*GP*T)-3')	polymer	6	1824.2	1		synthetic construct
5	E, R, S, U (A, b)	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)
6	F, G, H (A)	IODIDE ION	non-polymer		126.9	3	Chemie (IOD)
7	I, J, K, O (A)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
8	L, M, N, P, Q (A)	1,2-ETHANEDIOL	non-polymer		62.1	5	Chemie (EDO)
9	T, V (A, C)	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
10	W, X, Y, Z (A, B, b, C)	water	water		18.0	189	Chemie (HOH)

Sequence modifications

A: 59 - 196 (UniProt: Q9KEI9)

PDB	External Database	Details
Gly 55	-	expression tag
Ser 56	-	expression tag
His 57	-	expression tag
Met 58	-	expression tag
Ala 196	Lys 196	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	19926.8
Non-Polymers*	Number of molecules	18
	Total formula weight	1357.4
All*	Total formula weight	21284.2

*Water molecules are not included.