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6DPK

Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with an RNA/DNA Hybrid: Reaction in 4 mM Mn2+ and 200 mM K+ for 240 s at 21 C

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 22-BM
Synchrotron siteAPS
Beamline22-BM
Temperature [K]100
Detector technologyCCD
Collection date2015-04-24
DetectorMARMOSAIC 225 mm CCD
Wavelength(s)1
Spacegroup nameC 1 2 1
Unit cell lengths81.492, 37.598, 61.734
Unit cell angles90.00, 96.79, 90.00
Refinement procedure
Resolution19.920 - 1.391
R-factor0.1446
Rwork0.143
R-free0.16850
Starting model (for MR)pdbid 1ZBL
RMSD bond length0.008
RMSD bond angle1.088
Data reduction softwareHKL-2000
Data scaling softwareAimless (0.5.31)
Phasing softwarePHENIX
Refinement softwarePHENIX (1.10_2155)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]19.92019.9201.420
High resolution limit [Å]1.3907.4901.390
Total number of observations37271
Number of reflections372712251769
<I/σ(I)>6.7
Completeness [%]99.38692.9
Redundancy111
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP29414% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2

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