6DPK
Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with an RNA/DNA Hybrid: Reaction in 4 mM Mn2+ and 200 mM K+ for 240 s at 21 C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-24 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 81.492, 37.598, 61.734 |
Unit cell angles | 90.00, 96.79, 90.00 |
Refinement procedure
Resolution | 19.920 - 1.391 |
R-factor | 0.1446 |
Rwork | 0.143 |
R-free | 0.16850 |
Starting model (for MR) | pdbid 1ZBL |
RMSD bond length | 0.008 |
RMSD bond angle | 1.088 |
Data reduction software | HKL-2000 |
Data scaling software | Aimless (0.5.31) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.920 | 19.920 | 1.420 |
High resolution limit [Å] | 1.390 | 7.490 | 1.390 |
Total number of observations | 37271 | ||
Number of reflections | 37271 | 225 | 1769 |
<I/σ(I)> | 6.7 | ||
Completeness [%] | 99.3 | 86 | 92.9 |
Redundancy | 1 | 1 | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2 |