6DP8

Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 5 mM Mg2+ and 200 mM Li+ for 240 s at 21 C

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Ribonuclease H	polymer	142	16330.5	1	UniProt (Q9KEI9) In PDB	Bacillus halodurans	RNase H
2	B	RNA (5'-R(*AP*CP*AP*U)-3')	polymer	4	1224.8	1		synthetic construct
3	b	RNA (5'-R(P*CP*G)-3')	polymer	2	605.4	1		synthetic construct
4	C	DNA (5'-D(*CP*GP*AP*TP*GP*T)-3')	polymer	6	1824.2	1		synthetic construct
5	A, b	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	A	LITHIUM ION	non-polymer		6.9	1	Chemie (LI)
7	A	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
8	A	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
9	A, B	1,2-ETHANEDIOL	non-polymer		62.1	6	Chemie (EDO)
10	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
11		water	water		18.0	208	Chemie (HOH)

Sequence modifications

A: 59 - 196 (UniProt: Q9KEI9)

PDB	External Database	Details
Gly 55	-	expression tag
Ser 56	-	expression tag
His 57	-	expression tag
Met 58	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	19984.9
Non-Polymers*	Number of molecules	17
	Total formula weight	1022.8
All*	Total formula weight	21007.8

*Water molecules are not included.