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6DOW

Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 5 mM Mg2+ and 200 mM Rb+ for 160 s at 21 C

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A
(A)
Ribonuclease Hpolymer13615716.71UniProt (Q9KEI9)Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)RNase H
2B
(B)
5'-R(*AP*CP*AP*U)-3' portion of cleaved RNA 5'-R(*AP*CP*AP*UP*CP*G)-3'polymer41224.81synthetic construct
3C
(b)
5'-R(P*CP*G)-3' portion of cleaved RNA 5'-R(*AP*CP*AP*UP*CP*G)-3'polymer2605.41synthetic construct
4D
(C)
DNA (5'-D(*CP*GP*AP*TP*GP*T)-3')polymer61824.21synthetic construct
5E, F
(A)
MAGNESIUM IONnon-polymer24.32Chemie (MG)
6G, H, I, R, S
(A, B, C)
RUBIDIUM IONnon-polymer85.55Chemie (RB)
7J, K, L
(A)
CHLORIDE IONnon-polymer35.53Chemie (CL)
8M, N, O
(A)
GLYCEROLnon-polymer92.13Chemie (GOL)
9P, Q, T, U
(A, C)
1,2-ETHANEDIOLnon-polymer62.14Chemie (EDO)
10V, W, X, Y
(A, B, b, C)
waterwater18.0179Chemie (HOH)
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains4
Total formula weight19371.2
Non-Polymers*Number of molecules17
Total formula weight1106.9
All*Total formula weight20478.1
*Water molecules are not included.

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