6DOW
Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 5 mM Mg2+ and 200 mM Rb+ for 160 s at 21 C
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A (A) | Ribonuclease H | polymer | 136 | 15716.7 | 1 | UniProt (Q9KEI9) | Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) | RNase H |
2 | B (B) | 5'-R(*AP*CP*AP*U)-3' portion of cleaved RNA 5'-R(*AP*CP*AP*UP*CP*G)-3' | polymer | 4 | 1224.8 | 1 | synthetic construct | ||
3 | C (b) | 5'-R(P*CP*G)-3' portion of cleaved RNA 5'-R(*AP*CP*AP*UP*CP*G)-3' | polymer | 2 | 605.4 | 1 | synthetic construct | ||
4 | D (C) | DNA (5'-D(*CP*GP*AP*TP*GP*T)-3') | polymer | 6 | 1824.2 | 1 | synthetic construct | ||
5 | E, F (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
6 | G, H, I, R, S (A, B, C) | RUBIDIUM ION | non-polymer | 85.5 | 5 | Chemie (RB) | |||
7 | J, K, L (A) | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) | |||
8 | M, N, O (A) | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
9 | P, Q, T, U (A, C) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 4 | Chemie (EDO) | |||
10 | V, W, X, Y (A, B, b, C) | water | water | 18.0 | 179 | Chemie (HOH) |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 19371.2 | |
Non-Polymers* | Number of molecules | 17 |
Total formula weight | 1106.9 | |
All* | Total formula weight | 20478.1 |