6DF3

Crystal structure of ternary complex of IL-24 with soluble receptors IL-22RA and IL-20RB

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (L)	Interleukin-22 receptor subunit alpha-1	polymer	206	23560.8	1	UniProt (Q8N6P7) Pfam (PF01108)	Homo sapiens (Human)	IL-22RA1, Cytokine receptor class-II member 9, Cytokine receptor family 2 member 9, CRF2-9, ZcytoR11
2	B (C)	Interleukin-24	polymer	155	18202.0	1	UniProt (Q13007)	Homo sapiens (Human)	IL-24, Melanoma differentiation-associated gene 7 protein, MDA-7, Suppression of tumorigenicity 16 protein
3	C (H)	Interleukin-20 receptor subunit beta	polymer	190	21365.2	1	UniProt (Q6UXL0) Pfam (PF01108) Pfam (PF09294)	Homo sapiens (Human)	IL-20RB, Fibronectin type III domain containing 6, FNDC6, IL-20R2
4	D (A)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
5	E (L)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
6	F, G (C)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
7	H, I, J (L, C, H)	water	water		18.0	80	Chemie (HOH)

Sequence modifications

L: 24 - 228 (UniProt: Q8N6P7)

PDB	External Database	Details
Gly 229	-	expression tag

C: 52 - 206 (UniProt: Q13007)

PDB	External Database	Details
Gln 85	Asn 85	engineered mutation
Gln 99	Asn 99	engineered mutation
His 124	Tyr 124	conflict
Gln 126	Asn 126	engineered mutation

H: 35 - 224 (UniProt: Q6UXL0)

PDB	External Database	Details
Gln 134	Asn 134	engineered mutation
Glu 206	Gln 206	conflict

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	63128.0
Branched	Number of molecules	1
	Total formula weight	424.4
Non-Polymers*	Number of molecules	3
	Total formula weight	405.4
All*	Total formula weight	63957.8

*Water molecules are not included.