6DEJ

The structure of HcRed7, a brighter and red-shifted HcRed variant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	GFP-like non-fluorescent chromoprotein	polymer	237	26833.4	4	UniProt (Q95W85) Pfam (PF01353)	Heteractis crispa (Leathery sea anemone)	HcRed,hcCP
2	E, H (A, D)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
3	F (B)	DODECAETHYLENE GLYCOL	non-polymer		546.6	1	Chemie (12P)
4	G (D)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
5	I, J, K, L (A, B, C, D)	water	water		18.0	941	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 228 (UniProt: Q95W85)

PDB	External Database	Details
Met -8	-	initiating methionine
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag
Ala 37	Thr 36	engineered mutation
Cru 64	Glu 63	chromophore
Cru 64	Tyr 64	chromophore
Cru 64	Gly 65	chromophore
Lys 68	Arg 67	engineered mutation
His 123	Leu 122	engineered mutation
Ser 144	Cys 143	engineered mutation
His 169	Arg 168	engineered mutation
His 174	Leu 173	engineered mutation
Tyr 197	Ile 196	engineered mutation
Leu 202	Pro 201	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	107333.7
Non-Polymers*	Number of molecules	4
	Total formula weight	774.2
All*	Total formula weight	108107.9

*Water molecules are not included.