6DEJ

The structure of HcRed7, a brighter and red-shifted HcRed variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	54.342, 122.057, 75.254
Unit cell angles	90.00, 108.75, 90.00

Refinement procedure

Resolution	33.682 - 1.628
Rwork	0.178
R-free	0.21400
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.300	1.720
High resolution limit [Å]	1.628	1.628
Rmerge	0.093	1.554
Number of reflections	111329	13784
<I/σ(I)>	11.6	1.1
Completeness [%]	95.7	79.7
Redundancy	6.8	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	12 mg/ml protein 0.2 M ammonium sulfate 0.1 M bis-tris pH 6.5 25 w/v polyethylene glycol 3350