6DC6

Crystal structure of human ubiquitin activating enzyme E1 (Uba1) in complex with ubiquitin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Ubiquitin-like modifier-activating enzyme 1	polymer	1015	113504.7	2	UniProt (P22314) Pfam (PF00899) Pfam (PF16190) Pfam (PF16191) Pfam (PF10585) Pfam (PF09358)	Homo sapiens (Human)	Protein A1S9,Ubiquitin-activating enzyme E1
2	B, D (B, D)	Ubiquitin	polymer	80	8907.2	2	UniProt (P62979) Pfam (PF00240)	Homo sapiens (Human)
3	E, F, H, I (A, C)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
4	G, J (A, C)	PYROPHOSPHATE 2-	non-polymer		176.0	2	Chemie (POP)

Sequence modifications

A, C: 49 - 1058 (UniProt: P22314)

PDB	External Database	Details
Gly 44	-	expression tag
Ala 45	-	expression tag
Met 46	-	expression tag
Gly 47	-	expression tag
Ser 48	-	expression tag
Ala 632	Cys 632	engineered mutation

B, D: 1 - 76 (UniProt: P62979)

PDB	External Database	Details
Gly -3	-	expression tag
Ala -2	-	expression tag
Met -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	244823.8
Non-Polymers*	Number of molecules	6
	Total formula weight	449.1
All*	Total formula weight	245273.0

*Water molecules are not included.