6DC6
Crystal structure of human ubiquitin activating enzyme E1 (Uba1) in complex with ubiquitin
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000792 | cellular_component | heterochromatin |
| A | 0003723 | molecular_function | RNA binding |
| A | 0004839 | molecular_function | ubiquitin activating enzyme activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005654 | cellular_component | nucleoplasm |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005765 | cellular_component | lysosomal membrane |
| A | 0005829 | cellular_component | cytosol |
| A | 0006511 | biological_process | ubiquitin-dependent protein catabolic process |
| A | 0006974 | biological_process | DNA damage response |
| A | 0008641 | molecular_function | ubiquitin-like modifier activating enzyme activity |
| A | 0010008 | cellular_component | endosome membrane |
| A | 0016567 | biological_process | protein ubiquitination |
| A | 0016874 | molecular_function | ligase activity |
| A | 0030057 | cellular_component | desmosome |
| A | 0030867 | cellular_component | rough endoplasmic reticulum membrane |
| A | 0032446 | biological_process | protein modification by small protein conjugation |
| A | 0036211 | biological_process | protein modification process |
| A | 0070062 | cellular_component | extracellular exosome |
| C | 0000792 | cellular_component | heterochromatin |
| C | 0003723 | molecular_function | RNA binding |
| C | 0004839 | molecular_function | ubiquitin activating enzyme activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005524 | molecular_function | ATP binding |
| C | 0005634 | cellular_component | nucleus |
| C | 0005654 | cellular_component | nucleoplasm |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005739 | cellular_component | mitochondrion |
| C | 0005765 | cellular_component | lysosomal membrane |
| C | 0005829 | cellular_component | cytosol |
| C | 0006511 | biological_process | ubiquitin-dependent protein catabolic process |
| C | 0006974 | biological_process | DNA damage response |
| C | 0008641 | molecular_function | ubiquitin-like modifier activating enzyme activity |
| C | 0010008 | cellular_component | endosome membrane |
| C | 0016567 | biological_process | protein ubiquitination |
| C | 0016874 | molecular_function | ligase activity |
| C | 0030057 | cellular_component | desmosome |
| C | 0030867 | cellular_component | rough endoplasmic reticulum membrane |
| C | 0032446 | biological_process | protein modification by small protein conjugation |
| C | 0036211 | biological_process | protein modification process |
| C | 0070062 | cellular_component | extracellular exosome |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 2 |
| Details | binding site for residue MG A 1101 |
| Chain | Residue |
| A | ASP576 |
| A | POP1103 |
| site_id | AC2 |
| Number of Residues | 3 |
| Details | binding site for residue MG A 1102 |
| Chain | Residue |
| A | ASP506 |
| A | GLU509 |
| A | POP1103 |
| site_id | AC3 |
| Number of Residues | 9 |
| Details | binding site for residue POP A 1103 |
| Chain | Residue |
| A | GLN516 |
| A | LYS528 |
| A | ASP576 |
| A | MG1101 |
| A | MG1102 |
| A | ARG57 |
| A | ASP506 |
| A | ASN512 |
| A | ARG515 |
| site_id | AC4 |
| Number of Residues | 2 |
| Details | binding site for residue MG C 1101 |
| Chain | Residue |
| C | ASP576 |
| C | POP1103 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | binding site for residue MG C 1102 |
| Chain | Residue |
| C | ASP506 |
| C | GLU509 |
| C | LYS528 |
| C | POP1103 |
| site_id | AC6 |
| Number of Residues | 10 |
| Details | binding site for residue POP C 1103 |
| Chain | Residue |
| C | ARG57 |
| C | ASP506 |
| C | ASN512 |
| C | ARG515 |
| C | GLN516 |
| C | LYS528 |
| C | ASP576 |
| C | MG1101 |
| C | MG1102 |
| D | GLY76 |
Functional Information from PROSITE/UniProt
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | SITE: Interacts with activating enzyme |
| Chain | Residue | Details |
| B | ARG54 | |
| B | ARG72 | |
| D | ARG54 | |
| D | ARG72 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | SITE: Essential for function |
| Chain | Residue | Details |
| B | HIS68 | |
| C | ASP576 | |
| D | HIS68 | |
| A | ARG515 | |
| A | LYS528 | |
| A | ASP576 | |
| C | ALA478 | |
| C | ASP504 | |
| C | ARG515 | |
| C | LYS528 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine; by PINK1 => ECO:0000269|PubMed:24660806, ECO:0000269|PubMed:24751536, ECO:0000269|PubMed:24784582, ECO:0000269|PubMed:25527291 |
| Chain | Residue | Details |
| B | SER65 | |
| D | SER65 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | MOD_RES: (Microbial infection) ADP-ribosylthreonine => ECO:0000269|PubMed:32330457 |
| Chain | Residue | Details |
| B | THR66 | |
| D | THR66 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | MOD_RES: ADP-ribosylglycine => ECO:0000269|PubMed:28525742 |
| Chain | Residue | Details |
| B | GLY76 | |
| D | GLY76 | |
| C | LYS671 | |
| C | LYS980 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603 |
| Chain | Residue | Details |
| B | LYS6 | |
| D | LYS6 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 4 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins) |
| Chain | Residue | Details |
| A | SER810 | |
| B | GLY76 | |
| D | GLY76 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 4 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603, ECO:0000269|PubMed:16543144 |
| Chain | Residue | Details |
| B | LYS11 | |
| B | LYS48 | |
| D | LYS11 | |
| D | LYS48 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305|PubMed:15466860 |
| Chain | Residue | Details |
| B | LYS27 | |
| D | LYS27 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 2 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:25752573, ECO:0000269|PubMed:25752577, ECO:0000269|PubMed:34239127 |
| Chain | Residue | Details |
| B | LYS29 | |
| D | LYS29 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 2 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:25752577 |
| Chain | Residue | Details |
| B | LYS33 | |
| D | LYS33 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 2 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:18719106 |
| Chain | Residue | Details |
| B | LYS63 | |
| D | LYS63 |
