6D8P

Ternary RsAgo Complex Containing Guide RNA Paired with Target DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, B)	Uncharacterized protein	polymer	791	89038.4	2	UniProt (A4WYU7) Pfam (PF02171)	Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3)
2	B, E (E, C)	RNA (5'-R(P*UP*UP*AP*CP*UP*GP*CP*AP*CP*AP*GP*GP*UP*GP*AP*CP*GP*A)-3')	polymer	18	5772.5	2		Rhodobacter sphaeroides ATCC 17025
3	C, F (J, G)	DNA (5'-D(P*TP*CP*GP*TP*CP*AP*CP*CP*TP*GP*TP*GP*CP*AP*GP*TP*AP*AP*C)-3')	polymer	24	7336.7	2		Rhodobacter sphaeroides ATCC 17025
4	AA, G, H, I, J... (E, A, C)	MAGNESIUM ION	non-polymer		24.3	7	Chemie (MG)
5	BA, CA, DA, EA, FA... (E, J, B, A, G)	ACETATE ION	non-polymer		59.0	20	Chemie (ACT)
6	IA, X, Y, Z (B, A)	CACODYLATE ION	non-polymer		137.0	4	Chemie (CAC)
7	KA (C)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	1	Chemie (MPD)
8	MA, NA, OA, PA, QA... (A, E, J, B, C...)	water	water		18.0	720	Chemie (HOH)

Sequence modifications

A, B: 2 - 777 (UniProt: A4WYU7)

PDB	External Database	Details
Met -13	-	initiating methionine
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Asp -6	-	expression tag
Tyr -5	-	expression tag
Lys -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Asp 0	-	expression tag
Lys 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	204295.2
Non-Polymers*	Number of molecules	32
	Total formula weight	2017.1
All*	Total formula weight	206312.3

*Water molecules are not included.