6D83
Structure of the cargo bound AP-1:Arf1:tetherin-Nef (L164A, L165A) dileucine mutant dimer monomeric subunit
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | T, L | Bone marrow stromal antigen 2,Protein Nef | polymer | 264 | 29880.2 | 2 | UniProt (Q10589) UniProt (Q90VU7) Pfam (PF00469) In PDB | Homo sapiens (Human) | BST-2,HM1.24 antigen,Tetherin,3'ORF,Negative factor,F-protein |
2 | B | AP-1 complex subunit beta-1 | polymer | 586 | 66152.5 | 1 | UniProt (Q10567) Pfam (PF01602) In PDB | Homo sapiens (Human) | Adaptor protein complex AP-1 subunit beta-1,Adaptor-related protein complex 1 subunit beta-1,Beta-1-adaptin,Beta-adaptin 1,Clathrin assembly protein complex 1 beta large chain,Golgi adaptor HA1/AP1 adaptin beta subunit |
3 | C, H | ADP-ribosylation factor 1 | polymer | 193 | 22314.3 | 2 | UniProt (P84077) Pfam (PF00025) In PDB | Homo sapiens (Human) | |
4 | G | AP-1 complex subunit gamma-1 | polymer | 601 | 68194.1 | 1 | UniProt (P22892) Pfam (PF01602) In PDB | Mus musculus (Mouse) | Adaptor protein complex AP-1 subunit gamma-1,Adaptor-related protein complex 1 subunit gamma-1,Clathrin assembly protein complex 1 gamma-1 large chain,Gamma-adaptin,Gamma1-adaptin,Golgi adaptor HA1/AP1 adaptin subunit gamma-1 |
5 | M | AP-1 complex subunit mu-1 | polymer | 423 | 48606.7 | 1 | UniProt (P35585) Pfam (PF01217) Pfam (PF00928) In PDB | Mus musculus (Mouse) | AP-mu chain family member mu1A,Adaptor protein complex AP-1 subunit mu-1,Adaptor-related protein complex 1 subunit mu-1,Clathrin assembly protein complex 1 mu-1 medium chain 1,Clathrin coat assembly protein AP47,Clathrin coat-associated protein AP47,Golgi adaptor HA1/AP1 adaptin mu-1 subunit,Mu-adaptin 1,Mu1A-adaptin |
6 | S | AP-1 complex subunit sigma-3 | polymer | 154 | 18321.3 | 1 | UniProt (Q96PC3) Pfam (PF01217) In PDB | Homo sapiens (Human) | Adaptor protein complex AP-1 subunit sigma-1C,Adaptor-related protein complex 1 subunit sigma-1C,Clathrin assembly protein complex 1 sigma-1C small chain,Golgi adaptor HA1/AP1 adaptin sigma-1C subunit,Sigma 1C subunit of AP-1 clathrin,Sigma-adaptin 1C,Sigma1C-adaptin |
7 | C, H | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 2 | Chemie (GTP) | |||
8 | C, H | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) |
Sequence modifications
T, L: 2 - 21 (UniProt: Q10589)
T, L: 32 - 237 (UniProt: Q90VU7)
B: 1 - 584 (UniProt: Q10567)
C, H: 17 - 181 (UniProt: P84077)
G: 1 - 595 (UniProt: P22892)
S: 1 - 154 (UniProt: Q96PC3)
PDB | External Database | Details |
---|---|---|
Met -26 | - | expression tag |
Ser -25 | - | expression tag |
Tyr -24 | - | expression tag |
Tyr -23 | - | expression tag |
His -22 | - | expression tag |
His -21 | - | expression tag |
His -20 | - | expression tag |
His -19 | - | expression tag |
His -18 | - | expression tag |
His -17 | - | expression tag |
Asp -16 | - | expression tag |
Tyr -15 | - | expression tag |
Asp -14 | - | expression tag |
Ile -13 | - | expression tag |
Pro -12 | - | expression tag |
Thr -11 | - | expression tag |
Thr -10 | - | expression tag |
Glu -9 | - | expression tag |
Asn -8 | - | expression tag |
Leu -7 | - | expression tag |
Tyr -6 | - | expression tag |
Phe -5 | - | expression tag |
Gln -4 | - | expression tag |
Gly -3 | - | expression tag |
Ala -2 | - | expression tag |
Met -1 | - | expression tag |
Gly 0 | - | expression tag |
Ser 1 | - | expression tag |
Gly 22 | - | linker |
Ser 23 | - | linker |
Asp 24 | - | linker |
Glu 25 | - | linker |
Ala 26 | - | linker |
Ser 27 | - | linker |
Glu 28 | - | linker |
Gly 29 | - | linker |
Ser 30 | - | linker |
Gly 31 | - | linker |
PDB | External Database | Details |
---|---|---|
Ala 195 | Leu 164 | engineered mutation |
Ala 196 | Leu 165 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Gly -1 | - | expression tag |
Ser 0 | - | expression tag |
Arg 359 | Lys 359 | conflict |
Lys 476 | Glu 476 | conflict |
PDB | External Database | Details |
---|---|---|
Met -11 | - | expression tag |
Ser -10 | - | expression tag |
Tyr -9 | - | expression tag |
Tyr -8 | - | expression tag |
His -7 | - | expression tag |
His -6 | - | expression tag |
His -5 | - | expression tag |
His -4 | - | expression tag |
His -3 | - | expression tag |
His -2 | - | expression tag |
Asp -1 | - | expression tag |
Tyr 0 | - | expression tag |
Asp 1 | - | expression tag |
Ile 2 | - | expression tag |
Pro 3 | - | expression tag |
Thr 4 | - | expression tag |
Thr 5 | - | expression tag |
Glu 6 | - | expression tag |
Asn 7 | - | expression tag |
Leu 8 | - | expression tag |
Tyr 9 | - | expression tag |
Phe 10 | - | expression tag |
Gln 11 | - | expression tag |
Gly 12 | - | expression tag |
Ala 13 | - | expression tag |
Met 14 | - | expression tag |
Gly 15 | - | expression tag |
Ser 16 | - | expression tag |
Leu 71 | Gln 71 | conflict |
PDB | External Database | Details |
---|---|---|
Glu 596 | - | expression tag |
Asn 597 | - | expression tag |
Leu 598 | - | expression tag |
Tyr 599 | - | expression tag |
Phe 600 | - | expression tag |
Gln 601 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Cys 148 | Ser 148 | conflict |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 8 |
Total formula weight | 305663.5 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 1095.0 | |
All* | Total formula weight | 306758.5 |