6D7N

Crystal structure of the W357R/W399R Importin alpha mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B	Peroxidase,Importin subunit alpha-1	polymer	510	55272.5	1	UniProt (A0A1S4NYF8) UniProt (P52293) Pfam (PF01749) Pfam (PF00514) Pfam (PF16186) In PDB	Panicum virgatum (Switchgrass)	Importin alpha P1,Karyopherin subunit alpha-2,Pendulin,Pore targeting complex 58 kDa subunit,PTAC58,RAG cohort protein 1,SRP1-alpha
2	B	1,2-ETHANEDIOL	non-polymer		62.1	5	Chemie (EDO)
3		water	water		18.0	208	Chemie (HOH)

Sequence modifications

B: 37 - 57 (UniProt: A0A1S4NYF8)

PDB	External Database	Details
Met 20	-	initiating methionine
His 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
His 24	-	expression tag
His 25	-	expression tag
His 26	-	expression tag
Ser 27	-	expression tag
Ser 28	-	expression tag
Gly 29	-	expression tag
Leu 30	-	expression tag
Val 31	-	expression tag
Pro 32	-	expression tag
Arg 33	-	expression tag
Gly 34	-	expression tag
Ser 35	-	expression tag
Gly 36	-	expression tag
Asp 58	-	linker
Asp 59	-	linker
Asp 60	-	linker
Asp 61	-	linker
Lys 62	-	linker
Ala 63	-	linker
Met 64	-	linker
Ala 65	-	linker
Asp 66	-	linker
Ile 67	-	linker
Gly 68	-	linker
Ser 69	-	linker

B: 70 - 529 (UniProt: P52293)

PDB	External Database	Details
Arg 357	Trp 357	engineered mutation
Arg 399	Trp 399	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	55272.5
Non-Polymers*	Number of molecules	5
	Total formula weight	310.3
All*	Total formula weight	55582.9

*Water molecules are not included.