6D7N

Crystal structure of the W357R/W399R Importin alpha mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-28
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.514
Spacegroup name	P 21 21 21
Unit cell lengths	78.402, 90.637, 100.831
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.287 - 2.300
R-factor	0.1813
Rwork	0.179
R-free	0.21710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5e6q
RMSD bond length	0.011
RMSD bond angle	1.047
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.150
Rmeas	0.164	0.979
Rpim	0.064	0.395
Number of reflections	32563	1571
<I/σ(I)>	5.7	2.2
Completeness [%]	99.9	100
Redundancy	6.4	6.1
CC(1/2)		0.749

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	1.0M triammonium citrate, 0.1M Bis-Tris-Propane