6D6I

Ube2V1 in complex with ubiquitin variant Ubv.V1.1 and Ube2N/Ubc13

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	Ubiquitin-conjugating enzyme E2 variant 1	polymer	143	16138.5	2	UniProt (Q13404) Pfam (PF00179)	Homo sapiens (Human)	UEV-1,CROC-1,TRAF6-regulated IKK activator 1 beta Uev1A
2	B, E (B, E)	Ubiquitin-conjugating enzyme E2 N	polymer	155	17359.0	2	UniProt (P61088) Pfam (PF00179)	Homo sapiens (Human)	Bendless-like ubiquitin-conjugating enzyme,E2 ubiquitin-conjugating enzyme N,Ubc13,UbcH13,Ubiquitin carrier protein N,Ubiquitin-protein ligase N
3	C, F (C, F)	Ubv.V1.1	polymer	88	10044.3	2	UniProt (Q96H31) Pfam (PF00240)	Homo sapiens (Human)

Sequence modifications

A, D: 8 - 147 (UniProt: Q13404)

PDB	External Database	Details
Gly 5	-	expression tag
Ser 6	-	expression tag
Thr 7	-	expression tag

B, E: 3 - 152 (UniProt: P61088)

PDB	External Database	Details
Gly -2	-	expression tag
Ala -1	-	expression tag
Met 0	-	expression tag
Gly 1	-	expression tag
Ser 2	-	expression tag

C, F: 1 - 76 (UniProt: Q96H31)

PDB	External Database	Details
Gly -11	-	expression tag
Ala -10	-	expression tag
Gly -9	-	expression tag
Gly -8	-	expression tag
Asp -7	-	expression tag
Tyr -6	-	expression tag
Lys -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Lys 0	-	expression tag
His 62	Gln 75	engineered mutation
Trp 63	Lys 76	engineered mutation
Leu 68	His 81	engineered mutation
Trp 70	Val 83	engineered mutation
Trp 71	Leu 84	engineered mutation
Leu 74	Arg 87	engineered mutation
Ile 75	Gly 88	engineered mutation
Ala 76	Gly 89	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	87083.5
All*	Total formula weight	87083.5

*Water molecules are not included.