6D6I

Ube2V1 in complex with ubiquitin variant Ubv.V1.1 and Ube2N/Ubc13

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 32
Unit cell lengths	91.444, 91.444, 92.446
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.722 - 2.551
R-factor	0.2651
Rwork	0.264
R-free	0.29630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ait
Data reduction software	HKL-2000 (716.1)
Data scaling software	HKL-2000 (716.1)
Phasing software	PHASER (2.8.1)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.590
High resolution limit [Å]	2.550	6.920	2.550
Rmerge	0.065	0.049	0.950
Rmeas	0.072	0.054
Rpim	0.032	0.023	0.477
Total number of observations	143038
Number of reflections	28109	1416	1398
<I/σ(I)>	7.6
Completeness [%]	99.8	99.4	99.9
Redundancy	5.1	5.3	4.9
CC(1/2)		0.998	0.572

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.2M LiSO4, 25% PEG3350, 0.1 M Tris-HCl pH 8.5