6D68

Ube2G1 in complex with ubiquitin variant Ubv.G1.1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Ubiquitin-conjugating enzyme E2 G1	polymer	171	19698.4	2	UniProt (P62253) Pfam (PF00179)	Homo sapiens (Human)	E2 ubiquitin-conjugating enzyme G1,E217K,UBC7,Ubiquitin carrier protein G1,Ubiquitin-protein ligase G1
2	C, D (C, D)	Ubv.G1.1	polymer	86	9912.3	2	UniProt (Q96H31) Pfam (PF00240)	Homo sapiens (Human)
3	E, F, G, H (A, B, C, D)	water	water		18.0	85	Chemie (HOH)

Sequence modifications

A, B: 1 - 170 (UniProt: P62253)

PDB	External Database	Details
Ser 0	-	expression tag

C, D: 1 - 74 (UniProt: Q96H31)

PDB	External Database	Details
Gly -11	-	expression tag
Ala -10	-	expression tag
Gly -9	-	expression tag
Gly -8	-	expression tag
Asp -7	-	expression tag
Tyr -6	-	expression tag
Lys -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Lys 0	-	expression tag
Pro 7	Thr 20	engineered mutation
Ile 8	Leu 21	engineered mutation
Arg 9	Thr 22	engineered mutation
Val 10	Gly 23	engineered mutation
Ser 46	Ala 59	engineered mutation
Leu 49	Gln 62	engineered mutation
Tyr 65	Ser 78	engineered mutation
Leu 70	Val 83	engineered mutation
Arg 73	Leu 86	engineered mutation
His 74	Arg 87	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	59221.3
All*	Total formula weight	59221.3

*Water molecules are not included.