6D68

Ube2G1 in complex with ubiquitin variant Ubv.G1.1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 32 2 1
Unit cell lengths	66.537, 66.537, 225.098
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.520 - 2.360
R-factor	0.2147
Rwork	0.213
R-free	0.25360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2awf 1fxt
RMSD bond length	0.002
RMSD bond angle	0.538
Data reduction software	DENZO
Data scaling software	HKL-2000 (2.3.12)
Phasing software	PHASER (2.5.2)
Refinement software	PHENIX (phenix.refine: 1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.430
High resolution limit [Å]	2.350	5.060	2.350
Rmerge	0.105	0.084	0.403
Rmeas	0.114	0.092	0.439
Rpim	0.043	0.037	0.169
Total number of observations	168582
Number of reflections	24767	2681	2421
<I/σ(I)>	8
Completeness [%]	99.6	99.1	98.9
Redundancy	6.8	6.5	6.2
CC(1/2)		0.980	0.913

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	25% PEG1500, Succinic acid, Sodium phosphate monobasic monohydrate, Glycine