6D68
Ube2G1 in complex with ubiquitin variant Ubv.G1.1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-06 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 66.537, 66.537, 225.098 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.520 - 2.360 |
| R-factor | 0.2147 |
| Rwork | 0.213 |
| R-free | 0.25360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2awf 1fxt |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.538 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 (2.3.12) |
| Phasing software | PHASER (2.5.2) |
| Refinement software | PHENIX (phenix.refine: 1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 5.060 | 2.350 |
| Rmerge | 0.105 | 0.084 | 0.403 |
| Rmeas | 0.114 | 0.092 | 0.439 |
| Rpim | 0.043 | 0.037 | 0.169 |
| Total number of observations | 168582 | ||
| Number of reflections | 24767 | 2681 | 2421 |
| <I/σ(I)> | 8 | ||
| Completeness [%] | 99.6 | 99.1 | 98.9 |
| Redundancy | 6.8 | 6.5 | 6.2 |
| CC(1/2) | 0.980 | 0.913 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 25% PEG1500, Succinic acid, Sodium phosphate monobasic monohydrate, Glycine |
