6D24

Trypanosoma cruzi Glucose-6-P Dehydrogenase in complex with G6P

Replaces:  4EM5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Glucose-6-phosphate 1-dehydrogenase	polymer	541	60952.3	2	UniProt (Q1WBU6) Pfam (PF00479) Pfam (PF02781)	Trypanosoma cruzi
2	C, D (C, D)	Glucose-6-phosphate 1-dehydrogenase	polymer	541	60968.3	2	UniProt (Q1WBU6) Pfam (PF00479) Pfam (PF02781)	Trypanosoma cruzi
3	E, EA, R, UA (A, C, B, D)	6-O-phosphono-beta-D-glucopyranose	non-polymer		260.1	4	Chemie (BG6)
4	F, FA, G, GA, H... (A, C, B, D)	SULFATE ION	non-polymer		96.1	17	Chemie (SO4)
5	AB, BB, CB, DA, IA... (D, C, A, B)	GLYCEROL	non-polymer		92.1	14	Chemie (GOL)
6	AA, BA, CA, DB, EB... (B, D, A, C)	CHLORIDE ION	non-polymer		35.5	23	Chemie (CL)
7	KB, LB, MB, NB (A, B, C, D)	water	water		18.0	47	Chemie (HOH)

Sequence modifications

A, B: 38 - 555 (UniProt: Q1WBU6)

PDB	External Database	Details
Met 15	-	expression tag
Gly 16	-	expression tag
Ser 17	-	expression tag
Ser 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
His 24	-	expression tag
Ser 25	-	expression tag
Ser 26	-	expression tag
Gly 27	-	expression tag
Leu 28	-	expression tag
Val 29	-	expression tag
Pro 30	-	expression tag
Arg 31	-	expression tag
Gly 32	-	expression tag
Ser 33	-	expression tag
His 34	-	expression tag
Met 35	-	expression tag
Ala 36	-	expression tag
Ser 37	-	expression tag
Glu 290	Ala 290	engineered mutation

C, D: 38 - 555 (UniProt: Q1WBU6)

PDB	External Database	Details
Met 15	-	expression tag
Gly 16	-	expression tag
Ser 17	-	expression tag
Ser 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
His 24	-	expression tag
Ser 25	-	expression tag
Ser 26	-	expression tag
Gly 27	-	expression tag
Leu 28	-	expression tag
Val 29	-	expression tag
Pro 30	-	expression tag
Arg 31	-	expression tag
Gly 32	-	expression tag
Ser 33	-	expression tag
His 34	-	expression tag
Met 35	-	expression tag
Ala 36	-	expression tag
Ser 37	-	expression tag
Glu 290	Ala 290	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	243841.2
Non-Polymers*	Number of molecules	58
	Total formula weight	4778.4
All*	Total formula weight	248619.5

*Water molecules are not included.