Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 96.790, 133.030, 107.750 |
| Unit cell angles | 90.00, 100.27, 90.00 |
Refinement procedure
| Resolution | 34.160 - 3.350 |
| R-factor | 0.206 |
| Rwork | 0.205 |
| R-free | 0.25400 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.190 |
| Data reduction software | TRUNCATE |
| Data scaling software | SCALA (3.3.15) |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 67.359 | 67.359 | 3.530 |
| High resolution limit [Å] | 3.350 | 10.590 | 3.350 |
| Rmerge | 0.091 | 0.450 | |
| Rmeas | 0.246 | 0.107 | 0.530 |
| Rpim | 0.129 | 0.055 | 0.277 |
| Number of reflections | 38508 | 1276 | 5606 |
| <I/σ(I)> | 5.5 | 5.2 | 1.6 |
| Completeness [%] | 99.5 | 99.6 | 99.9 |
| Redundancy | 3.5 | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 4 microL 33 mg/mL protein (in 20 mM Tris pH 8.0, 50mM NaCl, 0.5mM MgCl2) plus 4 microL 4% PEG 400, 1.8 M ammonium sulfate, 0.1 M HEPES buffer, 5 mM G6P, pH 7.5 |
