6CMO

Rhodopsin-Gi complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	chimera protein of Soluble cytochrome b562 and Rhodopsin	polymer	463	52385.4	1	UniProt (P0ABE7) UniProt (P08100) Pfam (PF07361) Pfam (PF10413) Pfam (PF00001)	Escherichia coli	Cytochrome b-562,Opsin-2
2	B (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40445.1	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (Human)	Adenylate cyclase-inhibiting G alpha protein
3	C (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	345	37915.5	1	UniProt (P54311) Pfam (PF00400)	Rattus norvegicus (Rat)	Transducin beta chain 1
4	D (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	68	7563.8	1	UniProt (P63212) Pfam (PF00631)	Bos taurus (Bovine)	G gamma-I
5	E (L)	Fab light chain	polymer	215	23258.8	1		synthetic construct
6	F (H)	Fab Heavy chain	polymer	239	25788.8	1		synthetic construct
7	G (C)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)

Sequence modifications

R: -105 - -1 (UniProt: P0ABE7)

PDB	External Database	Details
Phe -139	-	expression tag
Ala -138	-	expression tag
Asp -137	-	expression tag
Tyr -136	-	expression tag
Lys -135	-	expression tag
Asp -134	-	expression tag
Asp -133	-	expression tag
Asp -132	-	expression tag
Asp -131	-	expression tag
Ala -130	-	expression tag
Lys -129	-	expression tag
Leu -128	-	expression tag
Gln -127	-	expression tag
Thr -126	-	expression tag
Met -125	-	expression tag
His -124	-	expression tag
His -123	-	expression tag
His -122	-	expression tag
His -121	-	expression tag
His -120	-	expression tag
His -119	-	expression tag
His -118	-	expression tag
His -117	-	expression tag
His -116	-	expression tag
His -115	-	expression tag
Glu -114	-	expression tag
Asn -113	-	expression tag
Leu -112	-	expression tag
Tyr -111	-	expression tag
Phe -110	-	expression tag
Gln -109	-	expression tag
Gly -108	-	expression tag
Gly -107	-	expression tag
Thr -106	-	expression tag
Trp -99	Met 29	conflict
Ile -4	His 124	conflict
Leu 0	-	linker
Met 1	-	linker
Cys 2	-	linker

R: 3 - 323 (UniProt: P08100)

PDB	External Database	Details
Gln 113	Glu 113	conflict
Tyr 257	Met 257	conflict
Cys 282	Asn 282	conflict

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Ala 203	Gly 203	conflict
Ser 326	Ala 326	conflict

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -4	-	initiating methionine
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	187357.3
Branched	Number of molecules	1
	Total formula weight	424.4
All*	Total formula weight	187781.7

*Water molecules are not included.