6CIM
Pre-Reaction Complex, RAG1(E962Q)/2-nicked/intact 12/23RSS complex in Mn2+
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C | V(D)J recombination-activating protein 1 | polymer | 625 | 71716.1 | 2 | UniProt (P15919) Pfam (PF12940) In PDB | Mus musculus (Mouse) | RAG-1 |
| 2 | B, D | V(D)J recombination-activating protein 2 | polymer | 359 | 40036.5 | 2 | UniProt (P21784) Pfam (PF03089) In PDB | Mus musculus (Mouse) | RAG-2 |
| 3 | N | High mobility group protein B1 | polymer | 163 | 18897.9 | 1 | UniProt (P09429) Pfam (PF09011) Pfam (PF00505) In PDB | Homo sapiens (Human) | High mobility group protein 1,HMG-1 |
| 4 | F | Nicked 12RSS intermediate reverse strand | polymer | 40 | 12430.0 | 1 | Homo sapiens | ||
| 5 | I | DNA (5'-D(*GP*CP*CP*TP*GP*TP*CP*TP*TP*A)-3') | polymer | 10 | 3011.0 | 1 | Homo sapiens | ||
| 6 | L | Nicked 12RSS intermediate forward strand | polymer | 30 | 9138.9 | 1 | Homo sapiens | ||
| 7 | G | Intact 23RSS substrate reverse strand | polymer | 56 | 17259.0 | 1 | Homo sapiens | ||
| 8 | J | Intact 23RSS substrate forward strand | polymer | 56 | 17251.1 | 1 | Homo sapiens | ||
| 9 | A, C | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 10 | A, C | MANGANESE (II) ION | non-polymer | 54.9 | 2 | Chemie (MN) | |||
| 11 | water | water | 18.0 | 3 | Chemie (HOH) |
Sequence modifications
A, C: 384 - 1008 (UniProt: P15919)
| PDB | External Database | Details |
|---|---|---|
| Gln 962 | Glu 962 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 301493.2 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 240.7 | |
| All* | Total formula weight | 301733.9 |
