6CIM
Pre-Reaction Complex, RAG1(E962Q)/2-nicked/intact 12/23RSS complex in Mn2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-02-16 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 156.678, 123.562, 186.286 |
Unit cell angles | 90.00, 105.60, 90.00 |
Refinement procedure
Resolution | 38.693 - 3.600 |
R-factor | 0.216 |
Rwork | 0.214 |
R-free | 0.25370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6cik |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.660 |
High resolution limit [Å] | 3.600 | 3.600 |
Rmerge | 0.119 | 0.999 |
Rmeas | 0.139 | 1.164 |
Rpim | 0.072 | 0.593 |
Number of reflections | 39753 | 1956 |
<I/σ(I)> | 14.6 | 1.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.8 | 3.8 |
CC(1/2) | 0.574 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 277 | 18% PEG3350, 200 mM KNO3, 50 mM HEPES pH 6.9 |