6CFZ

Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Ask1	polymer	77	8897.9	1	UniProt (G0S8F9) Pfam (PF08655) In PDB	Chaetomium thermophilum	DASH/Dam1 complex subunit Ask1
2	B	Dad3	polymer	67	7599.8	1	UniProt (G0RY74) Pfam (PF08656) In PDB	Chaetomium thermophilum	DASH/Dam1 complex subunit Dad3
3	C	Dad2	polymer	94	10229.7	1	UniProt (G0RZB3) Pfam (PF08654) In PDB	Chaetomium thermophilum	DASH/Dam1 complex subunit Dad2
4	D	Duo1	polymer	111	12907.6	1	UniProt (G0SAN7) Pfam (PF08651) In PDB	Chaetomium thermophilum	DASH/Dam1 complex subunit Duo1
5	E	Dad4	polymer	72	8400.6	1	UniProt (G0S589) Pfam (PF08650) In PDB	Chaetomium thermophilum	DASH/Dam1 complex subunit Dad4
6	F	Dad1,Dad1	polymer	92	10684.9	1	UniProt (G0RYE9) Pfam (PF08649) In PDB	Chaetomium thermophilum	DASH/Dam1 complex subunit Dad1
7	G	Hsk3	polymer	61	6641.7	1		Chaetomium thermophilum	DASH/Dam1 complex subunit Hsk3
8	H	Dam1	polymer	56	6314.1	1	UniProt (G0S2K4) Pfam (PF08653) In PDB	Chaetomium thermophilum	DASH/Dam1 complex subunit Dam1
9	I	Spc19	polymer	151	17028.4	1	UniProt (G0S0N0) Pfam (PF08287) In PDB	Chaetomium thermophilum	DASH/Dam1 complex subunit Spc19
10	J	Spc34	polymer	245	27443.8	1	UniProt (G0S2A3) Pfam (PF08657) In PDB	Chaetomium thermophilum	DASH/Dam1 complex subunit Spc34

Sequence modifications

A: 13 - 88 (UniProt: G0S8F9)

PDB	External Database	Details
Met 12	-	initiating methionine

B: 18 - 83 (UniProt: G0RY74)

PDB	External Database	Details
Met 17	-	initiating methionine

C: 25 - 117 (UniProt: G0RZB3)

PDB	External Database	Details
Met 24	-	initiating methionine

D: 49 - 158 (UniProt: G0SAN7)

PDB	External Database	Details
Met 48	-	initiating methionine

E: 1 - 63 (UniProt: G0S589)

PDB	External Database	Details
Ala 64	-	expression tag
Thr 65	-	expression tag
Asp 66	-	expression tag
Asn 67	-	expression tag
Leu 68	-	expression tag
Lys 69	-	expression tag
Asp 70	-	expression tag
Pro 71	-	expression tag
Ala 72	-	expression tag

F: 17 - 73 (UniProt: G0RYE9)
F: 74 - 108 (PDB: 6CFZ)
H: 53 - 107 (UniProt: G0S2K4)

PDB	External Database	Details
Met 52	-	initiating methionine

I: 7 - 156 (UniProt: G0S0N0)

PDB	External Database	Details
Met 6	-	initiating methionine

J: 1 - 239 (UniProt: G0S2A3)

PDB	External Database	Details
His 240	-	expression tag
His 241	-	expression tag
His 242	-	expression tag
His 243	-	expression tag
His 244	-	expression tag
His 245	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	116148.6
All*	Total formula weight	116148.6

*Water molecules are not included.