6CFZ
Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface
EM specimen
| Vitrification | Yes |
| Instrument | GATAN CRYOPLUNGE 3 |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.5 |
| Number of Particle | 34997 |
| Software | EMAN2,SPARX,cisTEM,Rosetta,PHENIX,SerialEM,Gctf,O |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
