6CBM

x-ray structure of NeoB from streptomyces fradiae in complex with PLP and neomycin (as the external aldimine) at pH 9

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Neamine transaminase NeoN	polymer	438	47165.0	2	UniProt (Q53U08) Pfam (PF00202) In PDB	Streptomyces fradiae	Glutamate--6'-dehydroparomamine aminotransferase,Neomycin C transaminase,Neomycin biosynthesis protein 18,Neo-18,Neomycin biosynthesis protein B,Neomycin biosynthesis protein N
2	A, B	(1R,2R,3S,4R,6S)-4,6-diamino-2-[(3-O-{2-amino-2,6-dideoxy-6-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]-alpha-D-glucopyranosyl}-beta-D-ribofuranosyl)oxy]-3-hydroxycyclohexyl 2,6-diamino-2,6-dideoxy-alpha-D-glucopyranoside	non-polymer		845.8	2	Chemie (OZY)
3	A, B	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
4	B	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
5		water	water		18.0	808	Chemie (HOH)

Sequence modifications

A, B: 1 - 416 (UniProt: Q53U08)

PDB	External Database	Details
Met -21	-	initiating methionine
Gly -20	-	expression tag
Ser -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	94329.9
Non-Polymers*	Number of molecules	6
	Total formula weight	1886.6
All*	Total formula weight	96216.5

*Water molecules are not included.