6C9A

Cryo-EM structure of mouse TPC1 channel in the PtdIns(3,5)P2-bound state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B, A	Two pore calcium channel protein 1	polymer	825	95414.8	2	UniProt (Q9EQJ0) Pfam (PF00520) In PDB	Mus musculus (Mouse)	Voltage-dependent calcium channel protein TPC1
2	C, D	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
3	B, A	(2R)-3-{[(S)-hydroxy{[(1S,2R,3R,4S,5S,6R)-2,4,6-trihydroxy-3,5-bis(phosphonooxy)cyclohexyl]oxy}phosphoryl]oxy}propane-1,2-diyl dioctanoate	non-polymer		746.6	2	Chemie (EUJ)
4	B, A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)

Sequence modifications

B, A: 1 - 817 (UniProt: Q9EQJ0)

PDB	External Database	Details
Val 818	-	expression tag
Glu 819	-	expression tag
Ala 820	-	expression tag
Gly 821	-	expression tag
Leu 822	-	expression tag
Val 823	-	expression tag
Pro 824	-	expression tag
Arg 825	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	190829.5
Branched	Number of molecules	2
	Total formula weight	848.8
Non-Polymers*	Number of molecules	4
	Total formula weight	1935.5
All*	Total formula weight	193613.9

*Water molecules are not included.