6C2Z

Crystal Structures of Cystathionine beta-Synthase from Saccharomyces cerevisiae: the Structure of the PLP-Aminoacrylate Intermediate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Cystathionine beta-synthase	polymer	375	41286.7	1	UniProt (P32582) Pfam (PF00291) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Beta-thionase, Serine sulfhydrase, Sulfur transfer protein 4
2	A	2-[({3-HYDROXY-2-METHYL-5-[(PHOSPHONOOXY)METHYL]PYRIDIN-4-YL}METHYL)AMINO]ACRYLIC ACID	non-polymer		318.2	1	Chemie (P1T)
3	A	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
4	A	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
5	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
7	A	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
8	A	1,2-ETHANEDIOL	non-polymer		62.1	7	Chemie (EDO)
9		water	water		18.0	350	Chemie (HOH)

Sequence modifications

A: 1 - 353 (UniProt: P32582)

PDB	External Database	Details
Met -21	-	expression tag
Gly -20	-	expression tag
Ser -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Asp -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	41286.7
Non-Polymers*	Number of molecules	14
	Total formula weight	1213.6
All*	Total formula weight	42500.4

*Water molecules are not included.