6C1D
High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E (A, B, C, D, E) | Actin, alpha skeletal muscle | polymer | 375 | 41862.6 | 5 | UniProt (P68135) Pfam (PF00022) | Oryctolagus cuniculus (Rabbit) | Alpha-actin-1 |
| 2 | F (P) | Unconventional myosin-Ib | polymer | 729 | 84143.9 | 1 | UniProt (Q05096) Pfam (PF00063) Pfam (PF00612) | Rattus norvegicus (Rat) | Myosin I alpha,MMIa,Myosin heavy chain myr 1 |
| 3 | G (R) | Calmodulin | polymer | 148 | 16721.3 | 1 | Pfam (PF13499) UniProt (by SIFTS) (P0DP23) | unidentified | |
| 4 | H (F) | Phalloidin | polymer | 7 | 808.9 | 1 | BIRD (PRD_002301) | Amanita phalloides (death cap) | |
| 5 | I, K, M, O, Q... (A, B, C, D, E...) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 6 | Chemie (ADP) | |||
| 6 | J, L, N, P, R... (A, B, C, D, E...) | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) |
Sequence modifications
P: 6 - 728 (UniProt: Q05096)
| PDB | External Database | Details |
|---|---|---|
| Gly 729 | - | expression tag |
| Leu 730 | - | expression tag |
| Asn 731 | - | expression tag |
| Asp 732 | - | expression tag |
| Ile 733 | - | expression tag |
| Phe 734 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 310987.2 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 2709.0 | |
| All* | Total formula weight | 313696.3 |
