6C1D

High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000287	molecular_function	magnesium ion binding
A	0001725	cellular_component	stress fiber
A	0003785	molecular_function	actin monomer binding
A	0005509	molecular_function	calcium ion binding
A	0005515	molecular_function	protein binding
A	0005523	molecular_function	tropomyosin binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005856	cellular_component	cytoskeleton
A	0005865	cellular_component	striated muscle thin filament
A	0005884	cellular_component	actin filament
A	0010628	biological_process	positive regulation of gene expression
A	0016787	molecular_function	hydrolase activity
A	0019904	molecular_function	protein domain specific binding
A	0030027	cellular_component	lamellipodium
A	0030041	biological_process	actin filament polymerization
A	0030175	cellular_component	filopodium
A	0030240	biological_process	skeletal muscle thin filament assembly
A	0031013	molecular_function	troponin I binding
A	0031432	molecular_function	titin binding
A	0031941	cellular_component	filamentous actin
A	0032036	molecular_function	myosin heavy chain binding
A	0032432	cellular_component	actin filament bundle
A	0042802	molecular_function	identical protein binding
A	0044297	cellular_component	cell body
A	0048306	molecular_function	calcium-dependent protein binding
A	0048741	biological_process	skeletal muscle fiber development
A	0051017	biological_process	actin filament bundle assembly
A	0090131	biological_process	mesenchyme migration
A	0098723	cellular_component	skeletal muscle myofibril
A	0140660	molecular_function	cytoskeletal motor activator activity
B	0000166	molecular_function	nucleotide binding
B	0000287	molecular_function	magnesium ion binding
B	0001725	cellular_component	stress fiber
B	0003785	molecular_function	actin monomer binding
B	0005509	molecular_function	calcium ion binding
B	0005515	molecular_function	protein binding
B	0005523	molecular_function	tropomyosin binding
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005856	cellular_component	cytoskeleton
B	0005865	cellular_component	striated muscle thin filament
B	0005884	cellular_component	actin filament
B	0010628	biological_process	positive regulation of gene expression
B	0016787	molecular_function	hydrolase activity
B	0019904	molecular_function	protein domain specific binding
B	0030027	cellular_component	lamellipodium
B	0030041	biological_process	actin filament polymerization
B	0030175	cellular_component	filopodium
B	0030240	biological_process	skeletal muscle thin filament assembly
B	0031013	molecular_function	troponin I binding
B	0031432	molecular_function	titin binding
B	0031941	cellular_component	filamentous actin
B	0032036	molecular_function	myosin heavy chain binding
B	0032432	cellular_component	actin filament bundle
B	0042802	molecular_function	identical protein binding
B	0044297	cellular_component	cell body
B	0048306	molecular_function	calcium-dependent protein binding
B	0048741	biological_process	skeletal muscle fiber development
B	0051017	biological_process	actin filament bundle assembly
B	0090131	biological_process	mesenchyme migration
B	0098723	cellular_component	skeletal muscle myofibril
B	0140660	molecular_function	cytoskeletal motor activator activity
C	0000166	molecular_function	nucleotide binding
C	0000287	molecular_function	magnesium ion binding
C	0001725	cellular_component	stress fiber
C	0003785	molecular_function	actin monomer binding
C	0005509	molecular_function	calcium ion binding
C	0005515	molecular_function	protein binding
C	0005523	molecular_function	tropomyosin binding
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005856	cellular_component	cytoskeleton
C	0005865	cellular_component	striated muscle thin filament
C	0005884	cellular_component	actin filament
C	0010628	biological_process	positive regulation of gene expression
C	0016787	molecular_function	hydrolase activity
C	0019904	molecular_function	protein domain specific binding
C	0030027	cellular_component	lamellipodium
C	0030041	biological_process	actin filament polymerization
C	0030175	cellular_component	filopodium
C	0030240	biological_process	skeletal muscle thin filament assembly
C	0031013	molecular_function	troponin I binding
C	0031432	molecular_function	titin binding
C	0031941	cellular_component	filamentous actin
C	0032036	molecular_function	myosin heavy chain binding
C	0032432	cellular_component	actin filament bundle
C	0042802	molecular_function	identical protein binding
C	0044297	cellular_component	cell body
C	0048306	molecular_function	calcium-dependent protein binding
C	0048741	biological_process	skeletal muscle fiber development
C	0051017	biological_process	actin filament bundle assembly
C	0090131	biological_process	mesenchyme migration
C	0098723	cellular_component	skeletal muscle myofibril
C	0140660	molecular_function	cytoskeletal motor activator activity
D	0000166	molecular_function	nucleotide binding
D	0000287	molecular_function	magnesium ion binding
D	0001725	cellular_component	stress fiber
D	0003785	molecular_function	actin monomer binding
D	0005509	molecular_function	calcium ion binding
D	0005515	molecular_function	protein binding
D	0005523	molecular_function	tropomyosin binding
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005856	cellular_component	cytoskeleton
D	0005865	cellular_component	striated muscle thin filament
D	0005884	cellular_component	actin filament
D	0010628	biological_process	positive regulation of gene expression
D	0016787	molecular_function	hydrolase activity
D	0019904	molecular_function	protein domain specific binding
D	0030027	cellular_component	lamellipodium
D	0030041	biological_process	actin filament polymerization
D	0030175	cellular_component	filopodium
D	0030240	biological_process	skeletal muscle thin filament assembly
D	0031013	molecular_function	troponin I binding
D	0031432	molecular_function	titin binding
D	0031941	cellular_component	filamentous actin
D	0032036	molecular_function	myosin heavy chain binding
D	0032432	cellular_component	actin filament bundle
D	0042802	molecular_function	identical protein binding
D	0044297	cellular_component	cell body
D	0048306	molecular_function	calcium-dependent protein binding
D	0048741	biological_process	skeletal muscle fiber development
D	0051017	biological_process	actin filament bundle assembly
D	0090131	biological_process	mesenchyme migration
D	0098723	cellular_component	skeletal muscle myofibril
D	0140660	molecular_function	cytoskeletal motor activator activity
E	0000166	molecular_function	nucleotide binding
E	0000287	molecular_function	magnesium ion binding
E	0001725	cellular_component	stress fiber
E	0003785	molecular_function	actin monomer binding
E	0005509	molecular_function	calcium ion binding
E	0005515	molecular_function	protein binding
E	0005523	molecular_function	tropomyosin binding
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0005856	cellular_component	cytoskeleton
E	0005865	cellular_component	striated muscle thin filament
E	0005884	cellular_component	actin filament
E	0010628	biological_process	positive regulation of gene expression
E	0016787	molecular_function	hydrolase activity
E	0019904	molecular_function	protein domain specific binding
E	0030027	cellular_component	lamellipodium
E	0030041	biological_process	actin filament polymerization
E	0030175	cellular_component	filopodium
E	0030240	biological_process	skeletal muscle thin filament assembly
E	0031013	molecular_function	troponin I binding
E	0031432	molecular_function	titin binding
E	0031941	cellular_component	filamentous actin
E	0032036	molecular_function	myosin heavy chain binding
E	0032432	cellular_component	actin filament bundle
E	0042802	molecular_function	identical protein binding
E	0044297	cellular_component	cell body
E	0048306	molecular_function	calcium-dependent protein binding
E	0048741	biological_process	skeletal muscle fiber development
E	0051017	biological_process	actin filament bundle assembly
E	0090131	biological_process	mesenchyme migration
E	0098723	cellular_component	skeletal muscle myofibril
E	0140660	molecular_function	cytoskeletal motor activator activity
P	0003774	molecular_function	cytoskeletal motor activity
P	0005524	molecular_function	ATP binding
P	0016459	cellular_component	myosin complex
R	0000086	biological_process	G2/M transition of mitotic cell cycle
R	0000922	cellular_component	spindle pole
R	0002027	biological_process	regulation of heart rate
R	0005509	molecular_function	calcium ion binding
R	0005513	biological_process	detection of calcium ion
R	0005515	molecular_function	protein binding
R	0005576	cellular_component	extracellular region
R	0005634	cellular_component	nucleus
R	0005654	cellular_component	nucleoplasm
R	0005737	cellular_component	cytoplasm
R	0005813	cellular_component	centrosome
R	0005819	cellular_component	spindle
R	0005829	cellular_component	cytosol
R	0005856	cellular_component	cytoskeleton
R	0005876	cellular_component	spindle microtubule
R	0005886	cellular_component	plasma membrane
R	0005929	cellular_component	cilium
R	0007186	biological_process	G protein-coupled receptor signaling pathway
R	0008076	cellular_component	voltage-gated potassium channel complex
R	0010800	biological_process	positive regulation of peptidyl-threonine phosphorylation
R	0010801	biological_process	negative regulation of peptidyl-threonine phosphorylation
R	0010856	molecular_function	adenylate cyclase activator activity
R	0010880	biological_process	regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
R	0010881	biological_process	regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
R	0016020	cellular_component	membrane
R	0016240	biological_process	autophagosome membrane docking
R	0019855	molecular_function	calcium channel inhibitor activity
R	0019901	molecular_function	protein kinase binding
R	0021762	biological_process	substantia nigra development
R	0030017	cellular_component	sarcomere
R	0030234	molecular_function	enzyme regulator activity
R	0031432	molecular_function	titin binding
R	0031514	cellular_component	motile cilium
R	0031954	biological_process	positive regulation of protein autophosphorylation
R	0031982	cellular_component	vesicle
R	0032465	biological_process	regulation of cytokinesis
R	0032516	biological_process	positive regulation of phosphoprotein phosphatase activity
R	0032991	cellular_component	protein-containing complex
R	0034704	cellular_component	calcium channel complex
R	0035307	biological_process	positive regulation of protein dephosphorylation
R	0035458	biological_process	cellular response to interferon-beta
R	0042995	cellular_component	cell projection
R	0043209	cellular_component	myelin sheath
R	0043539	molecular_function	protein serine/threonine kinase activator activity
R	0044325	molecular_function	transmembrane transporter binding
R	0046427	biological_process	positive regulation of receptor signaling pathway via JAK-STAT
R	0046872	molecular_function	metal ion binding
R	0048306	molecular_function	calcium-dependent protein binding
R	0050848	biological_process	regulation of calcium-mediated signaling
R	0051343	biological_process	positive regulation of cyclic-nucleotide phosphodiesterase activity
R	0051592	biological_process	response to calcium ion
R	0055117	biological_process	regulation of cardiac muscle contraction
R	0060314	biological_process	regulation of ryanodine-sensitive calcium-release channel activity
R	0060315	biological_process	negative regulation of ryanodine-sensitive calcium-release channel activity
R	0060316	biological_process	positive regulation of ryanodine-sensitive calcium-release channel activity
R	0071346	biological_process	cellular response to type II interferon
R	0071902	biological_process	positive regulation of protein serine/threonine kinase activity
R	0072542	molecular_function	protein phosphatase activator activity
R	0097225	cellular_component	sperm midpiece
R	0098901	biological_process	regulation of cardiac muscle cell action potential
R	0140056	biological_process	organelle localization by membrane tethering
R	1901842	biological_process	negative regulation of high voltage-gated calcium channel activity
R	1901844	biological_process	regulation of cell communication by electrical coupling involved in cardiac conduction
R	1902494	cellular_component	catalytic complex
R	1905913	biological_process	negative regulation of calcium ion export across plasma membrane
R	1990456	biological_process	mitochondrion-endoplasmic reticulum membrane tethering

Functional Information from PDB Data

site_id	AC1
Number of Residues	15
Details	binding site for residue ADP A 401

Chain	Residue
A	GLY13
A	GLU214
A	GLY302
A	THR303
A	MET305
A	LYS336
A	MG402
A	SER14
A	GLY15
A	LEU16
A	LYS18
A	GLY156
A	ASP157
A	ARG210
A	LYS213

---

site_id	AC2
Number of Residues	2
Details	binding site for residue MG A 402

Chain	Residue
A	GLN137
A	ADP401

---

site_id	AC3
Number of Residues	15
Details	binding site for residue ADP B 401

Chain	Residue
B	GLY13
B	SER14
B	GLY15
B	LEU16
B	LYS18
B	GLY156
B	ASP157
B	ARG210
B	LYS213
B	GLU214
B	GLY302
B	THR303
B	MET305
B	LYS336
B	MG402

---

site_id	AC4
Number of Residues	2
Details	binding site for residue MG B 402

Chain	Residue
B	GLN137
B	ADP401

---

site_id	AC5
Number of Residues	15
Details	binding site for residue ADP C 401

Chain	Residue
C	GLY13
C	SER14
C	GLY15
C	LEU16
C	LYS18
C	GLY156
C	ASP157
C	ARG210
C	LYS213
C	GLU214
C	GLY302
C	THR303
C	MET305
C	LYS336
C	MG402

---

site_id	AC6
Number of Residues	2
Details	binding site for residue MG C 402

Chain	Residue
C	GLN137
C	ADP401

---

site_id	AC7
Number of Residues	14
Details	binding site for residue ADP D 401

Chain	Residue
D	GLY13
D	SER14
D	GLY15
D	LEU16
D	LYS18
D	GLY156
D	ASP157
D	ARG210
D	LYS213
D	GLU214
D	GLY302
D	THR303
D	LYS336
D	MG402

---

site_id	AC8
Number of Residues	2
Details	binding site for residue MG D 402

Chain	Residue
D	GLN137
D	ADP401

---

site_id	AC9
Number of Residues	14
Details	binding site for residue ADP E 401

Chain	Residue
E	GLY13
E	SER14
E	GLY15
E	LEU16
E	LYS18
E	GLY156
E	ASP157
E	ARG210
E	LYS213
E	GLU214
E	GLY302
E	THR303
E	LYS336
E	MG402

---

site_id	AD1
Number of Residues	2
Details	binding site for residue MG E 402

Chain	Residue
E	GLN137
E	ADP401

---

site_id	AD2
Number of Residues	2
Details	binding site for residue MG P 801

Chain	Residue
P	THR115
P	ADP802

---

site_id	AD3
Number of Residues	2
Details	binding site for residue ADP P 802

Chain	Residue
P	ASN162
P	MG801

---

Functional Information from PROSITE/UniProt

site_id	PS00406
Number of Residues	11
Details	ACTINS_1 Actins signature 1. YVGDEAQs.KRG

Chain	Residue	Details
A	TYR53-GLY63

---

site_id	PS00018
Number of Residues	13
Details	EF_HAND_1 EF-hand calcium-binding domain. DKDGDGTITtkEL

Chain	Residue	Details
R	ASP20-LEU32
R	ASP56-PHE68
R	ASP93-LEU105
R	ASP129-PHE141

---

site_id	PS00432
Number of Residues	9
Details	ACTINS_2 Actins signature 2. WITKqEYDE

Chain	Residue	Details
A	TRP356-GLU364

---

site_id	PS01132
Number of Residues	13
Details	ACTINS_ACT_LIKE Actins and actin-related proteins signature. LLTEApLNPkaNR

Chain	Residue	Details
A	LEU104-ARG116

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	10
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00448, ECO:0000269|PubMed:1474585, ECO:0000269|PubMed:25441029, ECO:0000269|PubMed:27564677, ECO:0007744|PDB:1CLL, ECO:0007744|PDB:4UMO, ECO:0007744|PDB:4V0C, ECO:0007744|PDB:5J03

Chain	Residue	Details
R	ASP20
R	GLU67
R	ASP22
R	ASP24
R	THR26
R	GLU31
R	ASP56
R	ASP58
R	ASN60
R	THR62

---

site_id	SWS_FT_FI2
Number of Residues	10
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00448, ECO:0000269|PubMed:1474585, ECO:0000269|PubMed:27564677, ECO:0007744|PDB:1CLL, ECO:0007744|PDB:5J03

Chain	Residue	Details
R	ASP93
R	GLU140
R	ASP95
R	ASN97
R	TYR99
R	GLU104
R	ASP129
R	ASP131
R	ASP133
R	GLN135

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: N-acetylalanine => ECO:0000269|PubMed:7093203, ECO:0000269|Ref.7, ECO:0007744|PubMed:19413330, ECO:0007744|PubMed:22223895, ECO:0007744|PubMed:22814378, ECO:0007744|PubMed:25944712

Chain	Residue	Details
R	ALA1
B	LYS61
C	LYS61
D	LYS61
E	LYS61

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: N6-acetyllysine; alternate => ECO:0007744|PubMed:19608861

Chain	Residue	Details
R	LYS21
B	HIS73
C	HIS73
D	HIS73
E	HIS73

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: Phosphothreonine; by CaMK4 => ECO:0000250|UniProtKB:P0DP29

Chain	Residue	Details
R	THR44
B	LYS84
C	LYS84
D	LYS84
E	LYS84

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
R	SER81
B	ARG177
C	ARG177
D	ARG177
E	ARG177

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861

Chain	Residue	Details
R	LYS94

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19690332

Chain	Residue	Details
R	TYR99

---

site_id	SWS_FT_FI9
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569

Chain	Residue	Details
R	SER101

---

site_id	SWS_FT_FI10
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:24275569

Chain	Residue	Details
R	THR110

---

site_id	SWS_FT_FI11
Number of Residues	1
Details	MOD_RES: N6-methyllysine; alternate => ECO:0007744|PubMed:24129315

Chain	Residue	Details
R	LYS115

---

site_id	SWS_FT_FI12
Number of Residues	1
Details	MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:19690332

Chain	Residue	Details
R	TYR138

---

site_id	SWS_FT_FI13
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000250|UniProtKB:P62157

Chain	Residue	Details
R	LYS21

---