6C0B

Structural basis for recognition of frizzled proteins by Clostridium difficile toxin B

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Toxin B	polymer	522	59023.2	1	UniProt (M4NKV9) Pfam (PF12920) UniProt (by SIFTS) (P18177)	Clostridioides difficile (Peptoclostridium difficile)
2	B (B)	Frizzled-2	polymer	156	17447.8	1	UniProt (Q14332) Pfam (PF01392)	Homo sapiens (Human)	hFz2,FzE2
3	C, D, E (A)	MALONATE ION	non-polymer		102.0	3	Chemie (MLI)
4	F (B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
5	G (B)	PALMITOLEIC ACID	non-polymer		254.4	1	Chemie (PAM)
6	H, I (A, B)	water	water		18.0	37	Chemie (HOH)

Sequence modifications

A: 1285 - 1804 (UniProt: M4NKV9)

PDB	External Database	Details
Gly 1283	-	expression tag
Ser 1284	-	expression tag

B: 34 - 156 (UniProt: Q14332)

PDB	External Database	Details
His 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
Ser 10	-	expression tag
Gly 11	-	expression tag
Gly 12	-	expression tag
Gly 13	-	expression tag
Ser 14	-	expression tag
Gly 15	-	expression tag
Gly 16	-	expression tag
Gly 17	-	expression tag
Ile 18	-	expression tag
Glu 19	-	expression tag
Gly 20	-	expression tag
Arg 21	-	expression tag
Pro 22	-	expression tag
Ser 23	-	expression tag
Gly 24	-	expression tag
Ser 25	-	expression tag
Leu 26	-	expression tag
Glu 27	-	expression tag
Val 28	-	expression tag
Leu 29	-	expression tag
Phe 30	-	expression tag
Gln 31	-	expression tag
Gly 32	-	expression tag
Pro 33	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	76470.9
Non-Polymers*	Number of molecules	5
	Total formula weight	781.8
All*	Total formula weight	77252.7

*Water molecules are not included.