6C0B
Structural basis for recognition of frizzled proteins by Clostridium difficile toxin B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 74.611, 175.613, 174.423 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 87.806 - 2.500 |
| R-factor | 0.2006 |
| Rwork | 0.199 |
| R-free | 0.23740 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.017 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.860 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.047 | 0.595 |
| Number of reflections | 39399 | 3920 |
| <I/σ(I)> | 18.25 | |
| Completeness [%] | 98.0 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292.15 | 0.1 M sodium acetate (pH 5.0) and 1 M ammonium sulfate |
